The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis

Experimental Cell Research
K TanakaE A Nigg

Abstract

The human protein kinase Nek2 is related to the NIMA cell cycle regulatory kinase of Aspergillus nidulans. Whereas NIMA has been shown to be essential for cell cycle progression into mitosis in this fungus, the function of mammalian Nek2 remains to be elucidated. In this study, we isolated a cDNA coding for a mouse ortholog of human Nek2 and analyzed the expression of this kinase in different organs. RNase protection assays performed on RNAs from mouse adult organs showed very high expression of Nek2 in testis. Lower levels of transcripts were detected in intestine, thymus, and skin, three mitotically active organs, and whole-mount in situ hybridization performed on 10.5-day embryos allowed the detection of Nek2 transcripts in the brain. In situ hybridization analysis of testis sections revealed that the transcription of Nek2 occurred in a stage-specific manner during spermatogenesis. The strongest signals were seen in cells undergoing meiosis, but Nek2 transcripts could also be detected in haploid cells (stage I and II spermatids). Extending these results, in situ hybridization performed on ovary sections revealed strong signals in meiotically active oocytes. In addition, some Nek2 transcription was observed in actively dividi...Continue Reading

References

Jun 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E A Nigg
Oct 1, 1995·Current Biology : CB·A M Fry, E A Nigg
Jan 1, 1997·Methods in Enzymology·A M Fry, E A Nigg
Sep 1, 1964·The Journal of Cell Biology·V MONESI

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Citations

Jan 10, 2002·Genome Research·Reinhard HoffmannFritz Melchers
Jun 11, 2002·Biochemical and Biophysical Research Communications·Yoshihiro HashimotoMakoto Nakanishi
Apr 20, 2005·Oncogene·Maria A Ciemerych, Peter Sicinski
Aug 3, 2002·The Journal of Biological Chemistry·Kohji NoguchiYoshimasa Uehara
Jul 13, 2007·The Journal of Biological Chemistry·Wenjuan WuAndrew M Fry
Dec 18, 2013·Oncology Reports·Xia LiuFuqiang Yin
Jul 17, 1998·Experimental Cell Research·S GhoshD Schroeter
Feb 13, 2001·Biochemical and Biophysical Research Communications·T FujiokaT Uchida
Aug 26, 1998·Biochemical and Biophysical Research Communications·J ToshimaK Mizuno
Feb 19, 2003·Biochemical and Biophysical Research Communications·Shigeru MinoguchiAkihiko Yoshimura

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