The inability of Streptococcus mutans and Lactobacillus acidophilus to form a biofilm in vitro on dentine pretreated with ozone

Australian Dental Journal
G M KnightP S Zilm

Abstract

The use of ozone therapy in the treatment of dental caries is equivocal. The aim of this study was to use an in vitro model to determine the effects of prior ozone application to dentine on biofilm formation and to measure any associated reduction in bacteria viability. Twenty dentine discs were bonded to the bases of 5 mL polycarbonate screw top vials. Ten dentine discs were infused with ozone for 40 seconds, 10 samples remained untreated as a control. The vials were filled with nutrient medium, sterilized and placed into the outflow from a continuous chemostat culture of Streptococcus mutans and Lactobacillus acidophilus for four weeks. At the conclusion of the experiment bacterial growth was monitored by taking optical density readings of the growth medium in each vial and the outer surface of the dentine specimens were examined by scanning electron microscopy as shown by SEM analysis. Ozone infusion prevented biofilm formation on all the treated samples while there was substantial biofilm present on the control specimens. While the average optical density of the control specimens was almost twice that of the ozone infused dentine (0.710 for the control with a SD of 0.288 and 0.446 for the ozonated samples with a SD of 0.371...Continue Reading

References

Aug 1, 1991·Oral Microbiology and Immunology·A H RogersP D Marsh
May 22, 2004·Caries Research·E A M Kidd
Jun 24, 2004·Oral Microbiology and Immunology·M NagayoshiT Nishihara
Jul 22, 2004·The Cochrane Database of Systematic Reviews·G D RickardL Hooper
Oct 27, 2004·Journal of Dental Research·M TamesadaS Hamada
Oct 7, 2005·Evidence-based Dentistry·Dorothy McComb
Sep 26, 2006·Journal of Dental Research·A F Paes LemeJ A Cury
Dec 19, 2006·Journal of Dentistry·H K YipW H S Wong
Feb 20, 2007·European Journal of Oral Sciences·Philipp MüllerPatrick R Schmidlin

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Citations

Sep 15, 2011·Lasers in Medical Science·Evrim Eliguzeloglu DalkilicHuma Omurlu
Feb 28, 2013·Annals of Clinical Microbiology and Antimicrobials·May Lei MeiLakshman Perera Samaranayake
May 25, 2012·Operative Dentistry·R L QuockS A Patel
Aug 25, 2012·Journal of the Indian Society of Pedodontics and Preventive Dentistry·A AvinashR K Singh
Aug 24, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Merve Erkmen Almaz, Işıl Şaroğlu Sönmez
Apr 4, 2009·Journal of Dentistry·E JohanssonJ W V van Dijken
Oct 23, 2018·Stomatologii︠a︡·I M MakeevaM G Arakelyan
May 16, 2019·Stomatologii︠a︡·I M Makeeva, Ya V Samokhlib
Jan 1, 2010·The Journal of Clinical Pediatric Dentistry·S Burcak CehreliZafer Cavit Cehreli
Aug 31, 2019·International Journal of Microbiology·Santichai NunpanKornchanok Wayakanon
Mar 30, 2017·The Journal of Contemporary Dental Practice·Rafaella M ChavesLawrence G Lopes
May 29, 2021·Scientific Reports·Mahmoud K Al-OmiriEdward Lynch
Jun 20, 2021·Journal of Dentistry·John Yun NiuChun Hung Chu

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