The inactive 44-kDa processed form of membrane type 1 matrix metalloproteinase (MT1-MMP) enhances proteolytic activity via regulation of endocytosis of active MT1-MMP.

The Journal of Biological Chemistry
Jin-Ah ChoRafael Fridman

Abstract

Membrane type 1 (MT1) matrix metalloproteinase (MMP-14) is a membrane-tethered MMP considered to be a major mediator of pericellular proteolysis. MT1-MMP is regulated by a complex array of mechanisms, including processing and endocytosis that determine the pool of active proteases on the plasma membrane. Autocatalytic processing of active MT1-MMP generates an inactive membrane-tethered 44-kDa product (44-MT1) lacking the catalytic domain. This form preserves all other enzyme domains and is retained at the cell surface. Paradoxically, accumulation of the 44-kDa form has been associated with increased enzymatic activity. Here we report that expression of a recombinant 44-MT1 (Gly(285)-Val(582)) in HT1080 fibrosarcoma cells results in enhanced pro-MMP-2 activation, proliferation within a three-dimensional collagen I matrix, and tumor growth and lung metastasis in mice. Stimulation of pro-MMP-2 activation and growth in collagen I was also observed in other cell systems. Expression of 44-MT1 in HT1080 cells is associated with a delay in the rate of active MT1-MMP endocytosis resulting in higher levels of active enzyme at the cell surface. Consistently, deletion of the cytosolic domain obliterates the stimulatory effects of 44-MT1 on...Continue Reading

References

Jul 1, 1987·Molecular and Cellular Biology·T R FuerstB Moss
Nov 12, 1996·Biochemical and Biophysical Research Communications·D C GervasiR Fridman
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·Y T KonttinenM Seiki
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N ThéretB Clément
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M PréauxP Mavier
Feb 9, 2000·The Journal of Cell Biology·N KoshikawaV Quaranta
Apr 5, 2000·The Journal of Biological Chemistry·K LehtiJ Keski-Oja
Feb 15, 2001·The Biochemical Journal·B AnnabiR Béliveau
May 2, 2001·The Journal of Biological Chemistry·S M EllerbroekM S Stack
Dec 14, 2001·The Journal of Biological Chemistry·Boris I RatnikovAlex Y Strongin
May 25, 2002·Seminars in Cancer Biology·Sonia Hernandez-BarrantesRafael Fridman
Aug 27, 2002·The Journal of Biological Chemistry·Hidayatullah G MunshiM Sharon Stack
Nov 29, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nadia A HaseneenHussein D Foda
Dec 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Stanley ZuckerJian Cao
Apr 2, 2003·The Biochemical Journal·Erik MaquoiFrancis Frankenne
Apr 17, 2003·Current Topics in Developmental Biology·Stanley ZuckerCarlos Lopez-Otin
Dec 13, 2003·The Journal of Biological Chemistry·Yi I WuM Sharon Stack
Jan 20, 2004·Experimental Cell Research·Stanley ZuckerJian Cao
Feb 6, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Noriyuki InakiGo Watanabe
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric M TamChristopher M Overall

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Citations

Oct 13, 2010·The Journal of Biological Chemistry·Pameeka S Smith-PearsonAnn Marie Pendergast
Apr 24, 2012·Journal of Biomedical Science·Szu-Cheng KuoSzecheng J Lo
Feb 11, 2014·The Journal of Biological Chemistry·Hsueh-Liang FuRafael Fridman
Jan 9, 2016·Cell Adhesion & Migration·Alejandro Majali-MartinezMartina Dieber-Rotheneder
Jan 30, 2013·Biochemical and Biophysical Research Communications·Ju Hee LeeJeong-Heon Ko
Mar 3, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Panagiotis BourisNikos K Karamanos
May 5, 2009·Biochimica Et Biophysica Acta·Denis Gingras, Richard Béliveau
May 7, 2019·International Journal of Experimental Pathology·Marta BruczkoLech Romanowicz
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Farideh SabehStephen J Weiss
Dec 23, 2019·The Journal of Cell Biology·Kerrie B CollinsAndrei V Karginov
Mar 23, 2013·The Journal of Biological Chemistry·Hsueh-Liang FuRafael Fridman
Feb 8, 2020·Cells·Henar SuárezMaría Yáñez-Mó
Aug 21, 2009·Journal of Cell Science·Renaud PoinclouxPhilippe Chavrier
Jul 24, 2021·Journal of Molecular Cell Biology·Xiao-Dan XiaDa-Wei Zhang
Apr 10, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·S Pauliina TurunenKaisa Lehti

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