The incidence of a new human cross-reactive idiotype linked to subgroup VHIII heavy chains

Molecular Immunology
J J CrowleyD A Carson

Abstract

Cross-reactive idiotypes (CRI) on human rheumatoid factors (RF), which are identified by murine monoclonal antibodies (mAb), have proved useful in defining both the incidence and the structural characteristics of these autoantibodies. In this study, a new murine anti-idiotypic reagent, mAb B6, has been used to identify and define the expression of a distinct heavy chain CRI. The B6 CRI was found on 20% of monoclonal IgM (16 of 81), but on only 5% of monoclonal IgA (1 of 20) and on no monoclonal IgG. In addition, this CRI was expressed exclusively on a subset of Ig derived from the VHIII protein variable region subgroup. In immunoblotting experiments, the mAb B6 bound directly to the heavy (H) chains of CRI positive proteins. The B6 CRI was found frequently on monoclonal IgM-RF molecules, and the mAb B6 could inhibit the binding of the RF to its IgG antigen. It was also demonstrated that Staphylococcus aureus protein A (SpA), which has recently been shown to bind to the F(ab) region of VHIII molecules, could block the interaction of some B6 CRI positive IgM to the anti-CRI. These experiments suggest that the B6 CRI is a marker for one or a few VHIII genes and that it is expressed commonly on IgM paraproteins, many of which have ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1986·The Journal of Experimental Medicine·V RadouxD A Carson
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F R JirikD A Carson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P P ChenD A Carson
Dec 24, 1986·Journal of Immunological Methods·G J SilvermanS Fong
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T J KippsD A Carson
Nov 6, 1987·Science·H W SchroederR M Perlmutter
Dec 1, 1987·Arthritis and Rheumatism·D A CarsonS Fong
Feb 1, 1988·The Journal of Experimental Medicine·W B GraningerS J Korsmeyer
Aug 2, 1987·Nature·M J ShlomchikM G Weigert
Mar 29, 1985·Science·R M PerlmutterL E Hood
Feb 1, 1973·The Journal of Experimental Medicine·H G KunkelJ D Capra
May 1, 1970·Arthritis and Rheumatism·R W StevensH Gaafar
Sep 1, 1984·The Journal of Experimental Medicine·B Pons-EstelB Frangione

❮ Previous
Next ❯

Citations

Apr 1, 1995·Immunology Today·R Jefferis
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P M YangP P Chen
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·M BørretzenK M Thompson
Jul 1, 1991·The Journal of Experimental Medicine·R A MageedP M Lydyard
Apr 5, 1997·Annals of the New York Academy of Sciences·R A MageedJ B Natvig
Dec 15, 1996·The Journal of Clinical Investigation·P RobenG J Silverman
Apr 21, 1997·The Journal of Experimental Medicine·L Z Rassenti, T J Kipps
Jan 1, 1992·Rheumatology International·R MierauE Genth
Mar 14, 2007·Die Naturwissenschaften·Lee Alan Dugatkin
Jan 6, 2005·Springer Seminars in Immunopathology·Carl S Goodyear, Gregg J Silverman
May 4, 1992·Annals of the New York Academy of Sciences·P M LydyardR A Mageed
Nov 7, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L JuompanM Zouali
Jan 31, 2020·Frontiers in Oncology·Bruno Fattizzo, Wilma Barcellini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.