The incidence of Shiga toxin-producing Escherichia coli in cattle with mastitis in Brazil

Journal of Applied Microbiology
W M LiraJ M Marin

Abstract

To determine the prevalence and molecular characteristics of Shiga toxin-producing Escherichia coli (STEC) isolates from bovine mastitic milk in Brazil. A total of 2144 milk samples from dairy cattle showing mastitis were screened for the presence of E. coli. A total of 182 E. coli isolates were selected and examined. All were subjected to dot blot analysis using the CVD419 probe for the detection of the enterohaemolysin (hly) gene, and to a multiplex PCR for the detection of stx1, stx2 and eaeA genes. STEC were isolated from 22 (12.08%) milk samples. All the STEC isolates were tested for sensibility to 10 antimicrobials; the resistances most commonly observed were to cephalothin (86.3%), tetracycline (63.6%) and doxycycline (63.6%). STEC isolates were found in bovine mastitic milk in Brazil. STEC isolates from mastitic milk were potentially pathogenic for human in that they belonged to serogroups associated with diarrhoea and haemolytic-uraemic syndrome, some of them were stx2, eaeA and hly positive.

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Citations

Dec 5, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jeffrey T Lejeune, Päivi J Rajala-Schultz
Mar 1, 2014·Epidemiology and Infection·M Z IslamP K Biswas
Sep 4, 2012·International Journal of Food Microbiology·Choreh FarrokhOlivier Cerf
Jul 17, 2013·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Bruno Gomes de CastroAvelino José Bittencourt
Jan 10, 2019·Foodborne Pathogens and Disease·Shelton E MurindaKayla Osmon
Apr 25, 2018·Journal of Dairy Science·F DriehuisA T Adesogan
May 19, 2019·Microorganisms·Vinicius Silva CastroCarlos Adam Conte-Junior
Jan 1, 2017·Comprehensive Reviews in Food Science and Food Safety·E D van AsseltM Nierop Groot
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Vincenzina Fusco, Grazia Marina Quero

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