PMID: 8951431Nov 1, 1996Paper

The incorporation and turnover of radiolabelled amino acids in developing stereocilia of the chick cochlea

Hearing Research
J O PicklesG W Rouse

Abstract

Hair cell stereocilia are composed of packed actin filaments, oriented such that the preferred end for the addition of actin monomers is at the tips of the sterocilia. It has therefore been suggested that when stereocilia grow, they do so from their tips (Tilney and DeRosier, 1986, Dev. Biol. 116, 119-129). In order to test the hypothesis, radiolabelled amino acids were applied to the air-sac of chicken eggs at day 17 of incubation, i.e., at the beginning of a phase in which the stereocilia have achieved their mature width, but are growing rapidly in length. Incorporation of radiolabel was studied autoradiographically, followed by image analysis and averaging grain counts over many hair cells. In contrast to the position expected from the above hypothesis, there was no sign of preferential incorporation of label in the upper part of the stereociliary bundle. The greatest density of labelling was found in the lower part of the bundle, while the upper part of the bundle was under-represented in the autoradiographic averages. The turnover time (to fail to 1/e) was significantly greater in the bundle (16 days) than in the cuticular plate or in the rest of the cell (9 days). The results (i) give no support for the hypothesis that st...Continue Reading

References

Jul 1, 1992·Trends in Neurosciences·J O Pickles, D P Corey
Oct 5, 1989·Nature·A J Hudspeth
Mar 1, 1986·American Journal of Otolaryngology·D J Lim
Jan 1, 1986·Hearing Research·P R ThorneJ B Gavin
Aug 1, 1973·Journal of Nutritional Science and Vitaminology·R Funabiki, R G Cassens
Feb 1, 1984·The Journal of Cell Biology·R P StidwillD R Burgess
Jul 1, 1980·The Journal of Cell Biology·L G TilneyM J Mulroy
Jan 1, 1981·Archives of Oto-rhino-laryngology·H P Zenner
Jun 1, 1995·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·Y M SzymkoJ C Saunders
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Aug 23, 2002·Nature·Mark E SchneiderBechara Kachar
Oct 20, 1999·Developmental Biology·T SelfK P Steel

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