The incorporation of bFGF mediated by heparin into PCL/gelatin composite fiber meshes for guided bone regeneration

Drug Delivery and Translational Research
Ji-hye LeeHeungsoo Shin

Abstract

The concept of guided bone regeneration facilitated by barrier membranes has been widely considered to achieve enhanced bone healing in maxillofacial surgery. However, the currently available membranes are limited in their active regulation of cellular activities. In this study, we fabricated polycaprolactone/gelatin composite electrospun nanofibers incorporated with basic fibroblast growth factor (bFGF) to direct bone regeneration. The fibrous morphology was maintained after the crosslinking and subsequent conjugation of heparin. Release of bFGF from electrospun nanofibers without heparin resulted in a spontaneous burst, while the heparin-mediated release of bFGF decreased the burst release in 24 h. The bFGF released from the nanofibers enhanced the proliferation and migration of human mesenchymal stem cells as well as the tubule formation of human umbilical cord blood cells. The subcutaneous implantation of fibers incorporated with bFGF mobilized a large number of cells positive for CD31 and smooth muscle alpha actin within 2 weeks. The effect of the nanofibers incorporated with bFGF on bone regeneration was evaluated on a calvarial critical size defect model. As compared to the mice that received fibers without bFGF, which p...Continue Reading

References

Jan 1, 1992·Progress in Growth Factor Research·D LedouxD Barritault
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R MontesanoL Orci
Mar 1, 1994·The Journal of Surgical Research·N S GibranD Gordon
Feb 1, 1997·Endocrine Reviews·A BikfalviD B Rifkin
Feb 4, 1999·European Journal of Cell Biology·J L GirauxA M Fischer
Apr 12, 2002·Journal of Biomedical Materials Research·Wan-Ju LiFrank K Ko
May 27, 2004·Journal of Biomedical Materials Research. Part a·Chun-Hsu YaoChin-Chuan Tsai
Jul 12, 2005·Biomacromolecules·Cheryl L CasperJohn F Rabolt
Sep 8, 2005·Photomedicine and Laser Surgery·Marleny Elizabeth Márquez GerbiThais Oliveira Gonçalves
May 16, 2007·Journal of Biomedical Materials Research. Part a·Jun Jin YoonTae Gwan Park
Mar 31, 2009·Journal of Molecular and Cellular Cardiology·Harald F LangerMeinrad Gawaz
Sep 29, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Toru IshibeTetsu Takahashi
Dec 17, 2009·Journal of Biomedical Materials Research. Part a·Sambit SahooSiew-Lok Toh
Dec 15, 2010·Tissue Engineering. Part a·Jillian E TengoodSteven R Little
Sep 21, 2011·Biotechnology Journal·Piergiorgio GentileGianluca Ciardelli

❮ Previous
Next ❯

Citations

Aug 22, 2017·Tissue Engineering. Part C, Methods·Matthew P MurphyDerrick C Wan
Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing
Nov 17, 2017·Journal of Biomedical Materials Research. Part a·Elena Della BellaMilena Fini
May 7, 2020·Polymers·Fatemeh KhosraviMohammad Hossein Nasr-Esfahani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.