PMID: 5920195Apr 1, 1966

The incorporation of tritium from thymidine into proteins of the mouse

The Journal of Cell Biology
B J Bryant


Tritium from methyl-H(3)-thymidine was found to be incorporated into proteins in mice. This incorporation in the mouse as a whole represented between 1 and 10% of the injected tritium. Tritiated water was not an intermediate. Transmethylation reactions are proposed as a means whereby certain amino acids might have acquired the tritium from thymidine at some stage of its catabolism. The initial (2 hr) ratios of DNA to protein tritium activities per milligram of wet tissue ranged from 5 in two tissues of low DNA synthetic activity (pancreas, liver) to 35 to 40 in two tissues of high DNA synthetic activity (spleen, small intestine). Labeled nuclear protein was coincident with labeled DNA in nuclei, where it constituted less than 2.5% of the total tritium. The significance of the findings is discussed.


Aug 24, 1963·Nature·E A EVANS, F G STANFORD
Feb 1, 1965·Experimental Cell Research·B J Bryant
Jun 1, 1960·The Journal of Clinical Investigation·J R RUBINIT M FLIEDNER


May 3, 1976·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·D A Bosch, E J Ebels
Mar 1, 1973·Chemico-biological Interactions·D E Kizer, B A Howell
Oct 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·W W Morgan, S Mizell
Mar 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·W W Morgan, S Mizell
Jan 1, 1975·The Journal of Cell Biology·M L MeistrichW J Barcellona
Aug 1, 1969·Experimental Cell Research·J C SchaerR Schindler
Nov 1, 1967·Experimental Cell Research·A G Matthysse, J G Torrey
Aug 1, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H KorrC Schmitz
Jun 1, 1969·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·N O BianchiF N Dulout
Jan 10, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·V W PentreathE K Winstanley
Jan 1, 1967·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J F Scaife, H Brohée
Jan 1, 1976·Hereditas·K O Söderström, M Parvinen

Related Concepts

DNA, Double-Stranded
Intestines, Small
Protein Biosynthesis

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