PMID: 5920195Apr 1, 1966

The incorporation of tritium from thymidine into proteins of the mouse

The Journal of Cell Biology
B J Bryant

Abstract

Tritium from methyl-H(3)-thymidine was found to be incorporated into proteins in mice. This incorporation in the mouse as a whole represented between 1 and 10% of the injected tritium. Tritiated water was not an intermediate. Transmethylation reactions are proposed as a means whereby certain amino acids might have acquired the tritium from thymidine at some stage of its catabolism. The initial (2 hr) ratios of DNA to protein tritium activities per milligram of wet tissue ranged from 5 in two tissues of low DNA synthetic activity (pancreas, liver) to 35 to 40 in two tissues of high DNA synthetic activity (spleen, small intestine). Labeled nuclear protein was coincident with labeled DNA in nuclei, where it constituted less than 2.5% of the total tritium. The significance of the findings is discussed.

References

Aug 24, 1963·Nature·E A EVANS, F G STANFORD
Feb 1, 1965·Experimental Cell Research·B J Bryant
Jun 1, 1960·The Journal of Clinical Investigation·J R RUBINIT M FLIEDNER

Citations

May 3, 1976·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·D A Bosch, E J Ebels
Mar 1, 1973·Chemico-biological Interactions·D E Kizer, B A Howell
Oct 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·W W Morgan, S Mizell
Mar 1, 1971·Comparative Biochemistry and Physiology. A, Comparative Physiology·W W Morgan, S Mizell
Jan 1, 1975·The Journal of Cell Biology·M L MeistrichW J Barcellona
Aug 1, 1969·Experimental Cell Research·J C SchaerR Schindler
Nov 1, 1967·Experimental Cell Research·A G Matthysse, J G Torrey
Aug 1, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H KorrC Schmitz
Jun 1, 1969·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·N O BianchiF N Dulout
Jan 10, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·V W PentreathE K Winstanley
Jan 1, 1967·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·J F Scaife, H Brohée
Jan 1, 1976·Hereditas·K O Söderström, M Parvinen

Related Concepts

Autoradiography
DNA, Double-Stranded
Intestines, Small
Liver
Pancreas
Spleen
Thymidine
Protein Biosynthesis
Tritium

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.