The Increased Endogenous Sulfur Dioxide Acts as a Compensatory Mechanism for the Downregulated Endogenous Hydrogen Sulfide Pathway in the Endothelial Cell Inflammation

Frontiers in Immunology
Da ZhangHongfang Jin

Abstract

Endogenous hydrogen sulfide (H2S) and sulfur dioxide (SO2) are regarded as important regulators to control endothelial cell function and protect endothelial cell against various injuries. In our present study, we aimed to investigate the effect of endogenous H2S on the SO2 generation in the endothelial cells and explore its significance in the endothelial inflammation in vitro and in vivo. The human umbilical vein endothelial cell (HUVEC) line (EA.hy926), primary HUVECs, primary rat pulmonary artery endothelial cells (RPAECs), and purified aspartate aminotransferase (AAT) protein from pig heart were used for in vitro experiments. A rat model of monocrotaline (MCT)-induced pulmonary vascular inflammation was used for in vivo experiments. We found that endogenous H2S deficiency caused by cystathionine-γ-lyase (CSE) knockdown increased endogenous SO2 level in endothelial cells and enhanced the enzymatic activity of AAT, a major SO2 synthesis enzyme, without affecting the expressions of AAT1 and AAT2. While H2S donor could reverse the CSE knockdown-induced increase in the endogenous SO2 level and AAT activity. Moreover, H2S donor directly inhibited the activity of purified AAT protein, which was reversed by a thiol reductant DTT. M...Continue Reading

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Citations

Dec 7, 2018·Frontiers in Chemistry·Wenyi Wang, Binghe Wang
Jul 18, 2020·Journal of Cardiovascular Pharmacology·Dan ZhouYaqian Huang
Feb 24, 2020·Biochemical and Biophysical Research Communications·Yunjia SongHongfang Jin
Apr 13, 2021·Journal of Advanced Research·Lulu ZhangYaqian Huang
Jul 3, 2021·Cells·Lukas RoubenneChristelle Guibert
Jul 5, 2021·Biological Chemistry·Yolanda CorreiaKeqing Wang
Sep 21, 2021·Antioxidants & Redox Signaling·Yaqian HuangHongfang Jin
Sep 21, 2021·Experimental and Therapeutic Medicine·Maoxian Wang

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Methods Mentioned

BETA
nuclear translocation
electrophoresis
Enzyme-Linked Immunosorbent Assay
ELISA

Software Mentioned

SPSS
Image J

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