PMID: 9187025Apr 1, 1997Paper

The indirect fluorescent antibody test based on schizont antigen for study of the sheep parasite Theileria lestoquardi

Veterinary Parasitology
I LeemansA Uggla

Abstract

An indirect fluorescent antibody test (IFAT), based on schizont-infected lymphoblastoid cells, was applied to study the course of antibody production in adult sheep inoculated with attenuated, in vitro grown, Theileria lestoquardi (Theileria hirci) infected cells. Bright fluorescence of the intracellular schizonts could first be demonstrated 15 days after inoculation. A 32-64-fold rise in antibody titres was recorded 1 month after infection, and substantial titres were still observed 90 days after inoculation. Fluorescence was absent with negative control sera and background staining was minimal. No serological cross-reactions were detected with sheep sera positive for Babesia motasi, Babesia ovis or Toxoplasma gondii. Results obtained did not differ when antigens prepared from three different strains of T. lestoquardi infected lymphoid cells were compared. Testing for reactivity to non-pathogenic Theileria species of sheep revealed a low degree of cross-reaction of a Theileria ovis and a Theileria separata antiserum to T. lestoquardi antigen. Cross-reactions were also observed with bovine sera positive for Theileria annulata and Theileria parva. Moreover, T. lestoquardi positive sera reacted almost equally strongly with bovine...Continue Reading

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Citations

Jun 30, 2005·Parasitology Research·Eva SpitalskaOlivier A E Sparagano
Dec 6, 2007·Parasitology Research·Hong YinJabbar S Ahmed
Apr 4, 2008·Parasitology Research·Parviz ShayanSadegh Rahbari
Apr 26, 2000·Molecular and Biochemical Parasitology·R A SkiltonS P Morzaria
Feb 1, 2003·Veterinary Parasitology·D A SalihK M Taha
Dec 1, 2006·Annals of the New York Academy of Sciences·Mohammed A BakheitUlrike Seitzer
Dec 1, 2006·Annals of the New York Academy of Sciences·Jabbar S AhmedHong Yin
Jan 6, 2009·Annals of the New York Academy of Sciences·Mehdi NamavariOlivier A E Sparagano
Apr 17, 2016·Veterinary Parasitology·Oran ErsterVarda Shkap
Apr 2, 2015·International Journal for Parasitology. Parasites and Wildlife·Ben J MansAbdalla A Latif
Jun 13, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·M NamavariF Namazi
May 12, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Salama Al-HamidhiHamza A Babiker
Oct 28, 2016·Acta Parasitologica·Seyedeh Missagh JalaliAmeneh Darabifard

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