PMID: 9449251Aug 1, 1997Paper

The inducible and cytochrome P450-containing dehydroepiandrosterone 7alpha-hydroxylating enzyme system of Fusarium moniliforme

The Journal of Steroid Biochemistry and Molecular Biology
A C CotillonR Morfin

Abstract

7Alpha-hydroxylation of DHEA by Fusarium moniliforme was investigated with regard to inducibility and characterization of the responsible enzyme system. Using GC/MS, the 7-hydroxylated metabolites of DHEA produced after biotransformation by Fusarium moniliforme mycelia were identified. The strain of Fusarium moniliforme hydroxylated DHEA predominantly at the 7alpha-position, with minor hydroxylation occurring at the 7beta-position. Constitutive 7alpha-hydroxylation activity was low, but DHEA induced the enzyme complex responsible for 7alpha-hydroxylation via an increase in protein synthesis. DHEA 7alpha-hydroxylase was found to be mainly microsomal, and the best production yields of 7alpha-hydroxy-DHEA (28.5 +/- 3.51 pmol/min/mg protein) were obtained with microsomes prepared from 18-h-induced mycelia. Kinetic parameters (KM=1.18 +/- 0.035 microM and Vmax=909 +/- 27 pmol/min/mg protein) were determined. Carbon monoxide inhibited 7alpha-hydroxylation of DHEA by microsomes of Fusarium moniliforme. Also, exposure of mycelia to DHEA increased microsomal P450 content. These results demonstrated that: (i) DHEA is 7alpha-hydroxylated by microsomes of Fusarium moniliforme; (ii) DHEA induces Fusarium moniliforme 7alpha-hydroxylase; (iii...Continue Reading

References

Apr 1, 1978·Journal of Steroid Biochemistry·G DefayeE M Chambaz
Feb 1, 1978·Journal of Steroid Biochemistry·K Breskvar, T Hudnik-Plevnik
Aug 1, 1987·Journal of Steroid Biochemistry·M Zakelj-Mavric, I Belic
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 17, 1973·Biochimica Et Biophysica Acta·H Hafez-Zedan, R Oplourde
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Mar 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·K BreskvarT Hudnik-Plevnik
Feb 23, 1994·Molecular and Cellular Biochemistry·M KalimiW Regelson
Jul 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·R Morfin, G Courchay
Sep 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·G S BaillieF R Burnet
Jan 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·J DoostzadehR Morfin
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·G R JänigK Ruckpaul

❮ Previous
Next ❯

Citations

Jan 5, 2000·The Journal of Steroid Biochemistry and Molecular Biology·T Kołek
Dec 13, 2006·Drug Metabolism and Drug Interactions·Sonia Chalbot, Robert Morfin
Sep 29, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Katja Kristan, Tea Lanišnik Rižner
Feb 9, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Sae-Bom KimRobert Morfin
Jan 14, 2010·Prikladnaia biokhimiia i mikrobiologiia·T G LobastovaM V Donova
Jul 23, 1999·The Journal of Steroid Biochemistry and Molecular Biology·A C Cotillon, R Morfin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.