The induction of eosinophil peroxidase release: improved methods of measurement and stimulation

Journal of Immunological Methods
Darryl AdamkoRedwan Moqbel

Abstract

The production and release of eosinophil peroxidase (EPO) has been associated with human pathology. Degranulation assays with eosinophils are typically very difficult to do, with very low release values. EPO is unique for its high cationic charge. As such, it adheres to most extracellular surfaces, rendering it more difficult to measure compared with other released cellular proteins. Based on the understanding of the sticky nature of EPO, we were concerned that EPO released in vitro cannot be reproducibly measured in the supernatants of stimulated cells. Instead, we suspected that much of the released EPO was left adherent to the tube walls. We chose to investigate the measurement of EPO activity using the peroxidase substrate, O-phenylenediamine (OPD). Unlike other peroxidase substrates, OPD is soluble in aqueous physiological solutions, which do not lyse cell membranes, thereby allowing us to add OPD directly to eosinophils and exclusively measure extracellular EPO. This novel approach would remove the concerns of incorrect EPO measurements due to its adhesive nature. In addition, we developed this method to quantify EPO release in terms of EPO concentration. Finally, using this technique, we have been able to demonstrate sec...Continue Reading

References

Oct 11, 1990·The New England Journal of Medicine·J BousquetP Godard
Aug 1, 1996·The Journal of Allergy and Clinical Immunology·S HorieH Kita
Jun 6, 1998·American Journal of Respiratory Cell and Molecular Biology·M KatoH Kita
Oct 17, 1998·Journal of Immunological Methods·S Mahmudi-AzerR Moqbel
Apr 11, 2000·The Journal of Allergy and Clinical Immunology·G J Gleich
Sep 23, 2000·Japanese Journal of Pharmacology·Y KirinoC Kamei
Mar 15, 2002·Current Allergy and Asthma Reports·Darryl AdamkoRedwan Moqbel
Jun 18, 2003·Current Opinion in Pharmacology·Darryl AdamkoRedwan Moqbel

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Citations

May 7, 2011·Inflammation·Sonia Adi-BessalemFatima Laraba-Djebari
Jun 27, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Colleen S Curran, Paul J Bertics
Feb 2, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Revital ShamriPeter F Weller
Jun 1, 2006·International Archives of Allergy and Immunology·Francis DavoineMichel Laviolette
May 18, 2011·International Archives of Allergy and Immunology·Paige LacyNives Zimmermann
Feb 2, 2013·Journal of Inflammation·Francis DavoineRedwan Moqbel
Oct 5, 2010·Journal of Immunological Methods·Wonyoung KimByungsuk Kwon
May 6, 2008·The Journal of Allergy and Clinical Immunology·Darryl J AdamkoGerald J Gleich
Mar 1, 2013·Small·Fernando T AndónValerian E Kagan
Jan 5, 2014·British Journal of Haematology·Flavia Rubia PallisCarla Fernanda Franco-Penteado
Sep 18, 2009·Experimental Dermatology·Caroline M PercopoHelene F Rosenberg
Oct 28, 2014·Immunology·Solomon O OdemuyiwaPaige Lacy
May 13, 2008·The Journal of Allergy and Clinical Immunology·Francis DavoineDarryl J Adamko
Aug 30, 2013·Journal of Leukocyte Biology·John Dongil KimPaige Lacy
Oct 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Emily WoodhouseE J Milner-Gulland
Jul 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Noel Verjan GarciaMasayuki Miyasaka
Apr 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kimberly D DyerHelene F Rosenberg

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