The induction of thrombus generation on nanostructured neutral polymer brush surfaces

Biomaterials
Benjamin F L LaiJayachandran N Kizhakkedathu

Abstract

Surface induced thrombus generation is a major clinical concern associated with vascular medical devices and implants. Here, we show that high graft density hydrophilic non-charged poly (N,N-dimethylacrylamide) (PDMA) brushes prevent the initiation of blood coagulation on synthetic surfaces. Using a multi-faceted analysis approach, we have identified that PDMA brushes greater than 0.27 chains/nm(2) graft density showed this highly desired property. Non-specific protein adsorption is greatly reduced on high density brushes compared to bare surface as evident from isothermal titration calorimetry, gel electrophoresis, and proteomic analyses. We have identified approximately 129 proteins of various types on bare and PDMA brush coated surfaces at a range of surface concentrations. Thromboelastography, platelet activation, and aggregation analyses show that only high graft density brushes are neutral to blood coagulation. Since the polymer brush synthesis can be adapted to most currently used biomedical materials, these results have significant impact in the design of highly hemocompatible surfaces.

Citations

May 24, 2011·Seminars in Immunopathology·Werner Zimmerli, Parham Sendi
Apr 20, 2012·ACS Applied Materials & Interfaces·Bryan R CoadLaurence Meagher
Mar 9, 2011·Biomacromolecules·Cesar Rodriguez-EmmeneggerHynek Biederman
May 19, 2011·Biomacromolecules·Shaohui LinLi Jia
Sep 7, 2014·Colloids and Surfaces. B, Biointerfaces·Kai YuJayachandran N Kizhakkedathu
Oct 11, 2011·Advanced Drug Delivery Reviews·Sandrine DufortJean-Luc Coll
Jan 4, 2012·Journal of Molecular Recognition : JMR·Rajesh GhaiBrett M Collins
May 4, 2013·Chemistry, an Asian Journal·Basit Yameen, Aleeza Farrukh
Nov 28, 2012·Advanced Healthcare Materials·Kai YuJayachandran N Kizhakkedathu
Feb 14, 2015·Colloids and Surfaces. B, Biointerfaces·Chiaki YoshikawaEdith van den Bosch
Jul 29, 2015·Biomaterials Science·Christophe BlaszykowskiMichael Thompson
Mar 3, 2015·Biotechnology and Bioengineering·Eric OuelletCharles Haynes
May 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Kathryn A MelzakJosé L Toca-Herrera
Nov 8, 2016·Acta Biomaterialia·Kathryne S BrockmanJ Paul Santerre

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