PMID: 7541124Jun 1, 1995Paper

The induction of Trypanosoma cruzi trypomastigote to amastigote transformation by low pH

Parasitology
S TomlinsonV Nussenzweig

Abstract

Following cell invasion, Trypanosoma cruzi trypomastigotes transform into amastigotes, which are the mammalian replicative forms of the parasite. Although amastigotes represent a critical stage in the life-cycle of T. cruzi, little is known of the factors controlling trypomastigote to amastigote transformation. Kanbera et al. (1990) observed that exposure of trypomastigotes to acidic pH induced their transformation into rounded forms resembling amastigotes. We confirm their observation and, using two strains of T. cruzi, establish that these transformants are ultrastructurally and biochemically indistinguishable from natural amastigotes. Incubation of trypomastigotes in medium at pH 5.0 for 2 h was sufficient to trigger their transformation into forms resembling amastigotes. Electron microscopical analysis confirmed that the kinetoplast structure, and general morphological features of the acid-induced, extracellular amastigotes were indistinguishable from those of intracellular-derived amastigotes. The extracellular transformation was accompanied by the acquisition of the stage-specific surface antigen of the naturally transformed amastigotes (Ssp-4), and loss of a stage-specific trypomastigote antigen (Ssp-3). Trypomastigotes ...Continue Reading

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Citations

May 18, 2001·Journal of Insect Physiology·A H. KollienG A. Schaub
Sep 12, 2003·Molecular and Biochemical Parasitology·Todd A MinningRick L Tarleton
Jul 15, 1998·Molecular and Biochemical Parasitology·S RolinE Pays
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