The induction of tuftelin expression in PC12 cell line during hypoxia and NGF-induced differentiation

Journal of Cellular Physiology
Yoav LeiserDan Deutsch

Abstract

The tuftelin protein isoforms undergo post-translation modifications, and are ubiquitously expressed in various tissues in embryos, adults, and tumors. Developmental and pathological studies suggested an apparent correlation between oxygen deprivation and tuftelin expression. The aim of the study was therefore to investigate the effect of a pathological insult (hypoxia) and a physiological growth factor (NGF), which antagonistically regulate HIF1 expression, on tuftelin expression using the neuronal PC12 cell model. In the present study, we first demonstrated the expression of tuftelin in PC12 cells, providing an experimental system to investigate the pathophysiological role of tuftelin. Furthermore, we demonstrated the induction of tuftelin during hypoxia by oxygen deprivation and during chemical hypoxia by cobalt chloride. Down-regulation of HIF1α mRNA blocked hypoxia-induced HIF1α expression, and reduced by 89% hypoxia-induced tuftelin expression. In mice, intraperitoneal injection of cobalt chloride significantly induced tuftelin mRNA and protein expression in the brain. During NGF-mediated PC12 differentiation, tuftelin expression was significantly induced in correlation with neurite outgrowth. This induction was partially...Continue Reading

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Citations

Apr 1, 2014·Gynecologic Oncology·John K ChanDaniela Matei
Jan 25, 2012·European Journal of Oral Sciences·Dan DeutschAnat Blumenfeld
Sep 28, 2018·Journal of Neuroscience Research·Samantha PapalAnand Swaroop
Oct 3, 2017·PloS One·Miguel A S Martín-Aragón BaudelJennifer A Fraser
Mar 25, 2019·Journal of Molecular Neuroscience : MN·Dekel ShiloPhilip Lazarovici
Feb 10, 2018·Cell Discovery·Natsumi KawasakiDaizo Koinuma
May 3, 2019·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Dekel ShiloDan Deutsch
Sep 15, 2019·Histochemistry and Cell Biology·Jan BobekEva Matalova

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