The induction of tumor apoptosis in B16 melanoma following STAT3 siRNA delivery with a lipid-substituted polyethylenimine

Biomaterials
Aws AlshamsanHasan Uludağ

Abstract

Persistent activation of signal transducer and activator of transcription 3 (STAT3) has been shown to impart several oncogenic features in many solid and blood tumors. In this study, we investigated the potential of nanoparticles based on polyethylenimine (PEI) modified with stearic acid (StA), to deliver siRNA for efficient STAT3 downregulation in B16 melanoma cells. The B16 cells were targeted with approximately 6-200 nm of siRNA complexes for 36 h. Compared to the PEI complexes, the PEI-StA complexes showed higher potency in STAT3 silencing in B16 cells accompanied by a significant induction of IL-6 secretion and a reduction of VEGF production. Moreover, with PEI-StA complexes, the level of the cellular Caspase 3 activity (an indicator of apoptotic activity) was found to be 2.5 times higher than that of PEI complexes. When the cells were treated with 50 nm of siRNA complexes on a daily basis, the cell viability was dramatically reduced reaching only to 16% after the third daily dose of PEI-StA complexes, as compared to the 69% viability observed with the PEI complexes at an equivalent time period. Consistently, in vivo results indicated significant regression in tumor growth and tumor weight after siRNA/PEI-StA treatment as ...Continue Reading

References

Jul 9, 1999·Journal of Biomedical Materials Research·W T GodbeyA G Mikos
Jul 15, 1999·Biochemical and Biophysical Research Communications·C CostanzoM Palmieri
Dec 21, 2002·Science·Donald Kennedy
Feb 18, 2004·Nature Reviews. Cancer·Hua Yu, Richard Jove
Jun 3, 2005·Anti-cancer Drugs·Naijie Jing, David J Tweardy
Jun 7, 2005·Nature Medicine·James E Darnell
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·U LungwitzA Göpferich
Aug 24, 2005·FEBS Letters·Susan L Uprichard
Dec 13, 2005·Clinical Science·Daniel CejkaVolker Wacheck
Feb 17, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mark A Behlke
Apr 14, 2006·Current Drug Delivery·Ian R GilmoreSaghir Akhtar
Jul 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sun Hwa KimTae Gwan Park
Sep 6, 2006·Critical Reviews in Therapeutic Drug Carrier Systems·D Putnam, A Doody
Feb 20, 2007·Current Cancer Drug Targets·Jinxia DengNouri Neamati
Mar 7, 2007·Journal of Biomedical Materials Research. Part a·Vanessa IncaniHasan Uludag
Apr 26, 2007·Medicinal Research Reviews·Zhong Chen, Zhong Chao Han
Apr 27, 2007·Advanced Drug Delivery Reviews·Mini ThomasAlexander M Klibanov
Jan 30, 2008·Cell Research·Khandaker Al Zaid Siddiquee, James Turkson
Mar 28, 2008·Cell Research·Jinbo Yang, George R Stark
May 20, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sun Hwa KimTae Gwan Park
Jul 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Navneet DhillonRazelle Kurzrock
Dec 5, 2008·Molecular Pharmaceutics·Aws AlshamsanHasan Uludağ
Dec 5, 2008·Expert Opinion on Investigational Drugs·Peibin Yue, James Turkson
Apr 9, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joseph A SparanoSaid M Sebti

❮ Previous
Next ❯

Citations

Nov 3, 2011·Expert Reviews in Molecular Medicine·Ivan PachecoVictor Tron
Jul 14, 2011·Nucleic Acid Therapeutics·Kaushik SinghaWon Jong Kim
Nov 13, 2013·Immunology and Cell Biology·Norliana KhairuddinNigel A J McMillan
Sep 16, 2014·Journal of Pharmaceutical Innovation·Yi Wen, Wilson S Meng
Jan 17, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Shuo-Li SunLi-Fang Wang
Mar 19, 2011·Expert Opinion on Drug Delivery·Xudong YuanZhiqian Wu
Aug 11, 2012·Expert Opinion on Investigational Drugs·Diwakar Davar, John Munn Kirkwood
Dec 21, 2012·Expert Opinion on Drug Delivery·Soma Patnaik, Kailash C Gupta
Jul 5, 2012·Journal of Drug Targeting·Zong-Wei WuShu-Yi Lin
Feb 15, 2011·Expert Opinion on Drug Delivery·Ezharul Hoque Chowdhury
Jun 5, 2012·Expert Opinion on Drug Delivery·Yitzhak RosenNoel M Elman
Aug 29, 2012·Journal of Pharmaceutical Sciences·Diana Guzman-VillanuevaHugh D C Smyth
Feb 8, 2011·Advanced Drug Delivery Reviews·Omid VeisehMiqin Zhang
Dec 1, 2010·Nano Today·Gang LiuXiaoyuan Chen
Oct 16, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sabrina HöbelAchim Aigner
Aug 6, 2010·The Journal of Investigative Dermatology·Yunching ChenLeaf Huang
Dec 8, 2015·Nano Today·Alexander S Cheung, David J Mooney
May 30, 2012·Advanced Materials·James J MoonDarrell J Irvine
May 31, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Sabrina Höbel, Achim Aigner
Nov 22, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Wei ChenTingbo Liang
Feb 23, 2011·Chemistry : a European Journal·Melissa D Krebs, Eben Alsberg
Feb 14, 2015·Colloids and Surfaces. B, Biointerfaces·Yunchun ZhaoMengdan Zhao
Sep 16, 2015·Molecular Therapy. Nucleic Acids·Jenny K W LamSusan W S Leung
Jul 16, 2015·Human Gene Therapy·Ling LiYu-Quan Wei
Jun 20, 2016·Colloids and Surfaces. B, Biointerfaces·Suman LabalaVenkata Vamsi Krishna Venuganti
Jul 5, 2016·Biochimica Et Biophysica Acta·Kheshwant S GillPatrick F Forde
Nov 3, 2016·ACS Applied Materials & Interfaces·Lisi XieHairong Zheng
Nov 8, 2016·Journal of Drug Targeting·Anup JoseVenkata Vamsi Krishna Venuganti
Apr 21, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Aishwarya SinghNarendra Kumar Jain
Mar 25, 2017·International Journal of Cancer. Journal International Du Cancer·Jian-Qin TangGuan Jiang
Jan 1, 2010·Cancers·Stephen J BeebeRichard Heller
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jiahe LiPaula T Hammond
Mar 27, 2018·Annual Review of Chemical and Biomolecular Engineering·Johan KarlssonJordan J Green
Jan 20, 2018·Scientific Reports·Jingtong PanYuehong Xu

❮ Previous
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