The inductive role of ionic binding in the bactericidal and postexposure effects of aminoglycoside antibiotics with implications for dosing

The Journal of Infectious Diseases
G G JacksonG L Daikos

Abstract

Gram-negative bacilli precooled to 4 degrees C to inactivate energy-dependent drug transport were exposed to an aminoglycoside antibiotic to assess the antibacterial effect of passive ionic binding of drug. Removal of free drug and energizing the cells by incubation at 37 degrees C showed the postexposure effect to be bactericidal. The effect was directly related to the amount and concentration of drug in the initial exposure medium proportional to the bacterial density. Binding was nonsaturable at the highest drug:bacteria ratio tested. Elution, exposure of spheroplasts, and inhibition by divalent cations indicated binding sites in the outer bacterial membrane. Different bacterial species had variable efficiency but similar patterns of binding different aminoglycosides reflecting in vitro susceptibility. The self-promoted postexposure internalization of ionically bound aminoglycoside accounts for the early drug-concentration-dependent rapid bactericidal action of aminoglycosides. The phenomenon has implications for effective initial dosing with aminoglycoside antibiotics.

Citations

Jun 1, 1996·Obstetrics and Gynecology·G Del PrioreM C Frederiksen
Aug 11, 2010·Antimicrobial Agents and Chemotherapy·Wendy RibbleJames M Bullard
May 1, 1994·Antimicrobial Agents and Chemotherapy·M StanevaD Terziivanov
Nov 29, 2011·International Journal of Otolaryngology·M E HuthA G Cheng
Jan 31, 2014·Annals of the American Thoracic Society·Jerry A NickDavid P Nichols
Nov 13, 2004·Intensive Care Medicine·Rina MehrotraMark Palazzo
Jul 1, 1997·The Journal of Pediatrics·K C HayaniD Vidyasagar
Jul 1, 1995·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M AntosJ Grzybowski
Nov 21, 2001·Pharmacotherapy·B W GundersonJ C Rotschafer
Jan 14, 2003·Pharmacotherapy·Chad A KnodererWilliam F Buss
Apr 20, 2000·American Journal of Veterinary Research·T B LescunR P Bill
Apr 3, 2016·Critical Care Nurse·Molly E DroegeEric W Mueller
Jun 1, 1994·The Annals of Pharmacotherapy·J C Rotschafer, M J Rybak
Sep 1, 1993·Journal of Bacteriology·J L KadurugamuwaT J Beveridge

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Intensive & Critical Care Nursing : the Official Journal of the British Association of Critical Care Nurses
A M MacConnachie
ORL; Journal for Oto-rhino-laryngology and Its Related Specialties
Masaya Takumida, Matti Anniko
© 2021 Meta ULC. All rights reserved