The influence of alpha-amanitin on the NaCl-induced up-regulation of antioxidant enzyme activity in cotton callus tissue

Free Radical Research
A M ManchandiaE. P. Millhollon

Abstract

Liquid suspensions of cotton callus tissue from a NaCl-sensitive cell line and a NaCl-tolerant cell line were subjected to the following treatments: (a) 0 and 150 mM NaCl, respectively (controls); (b) 75 and 250 mM NaCl, respectively; (c) 100 ng ml(-1) alpha-amanitin; or (d) pretreatment for 2 h with 100 ng ml(-1) alpha-amanitin followed by the respective NaCl treatments. The callus tissue was harvested at 0, 0.5, 1, 2, 4, and 8h and analyzed for antioxidant enzyme activity. In the NaCl-tolerant callus, the 250 mM NaCl treatment resulted in transient 2- to 4-fold increases above the control levels in the activities of ascorbate peroxidase, catalase, glutathione reductase, and peroxidase within 1 h after treatment, while superoxide dismutase activity increased 4-fold within 4 h. This rapid increase suggests that the up-regulation of antioxidant capacity is an early response to NaCl stress and perhaps provides protection against oxidative damage until other acclimating mechanisms can be invoked. In the control callus, peroxidase activity remained unchanged, and significant increases in the other enzymes were not observed until 8 h after treatment with 75mM NaCl. Pre-treatment with alpha-amanitin prior to the NaCl treatment comple...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D Williamson, J G Scandalios
Sep 1, 1973·Archives of Biochemistry and Biophysics·H J Forman, I Fridovich
Jun 27, 1974·Biochimica Et Biophysica Acta·M Cochet-Meilhac, P Chambon
Feb 5, 1998·Plant Physiology·K D RichardsR C Gardner
Aug 1, 1994·Plant Physiology·Y. K. Sharma, K. R. Davis
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D N KuhnK Hahlbrock
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K EdwardsC J Lamb
May 1, 1977·Plant Physiology·M Schaedle
Jun 1, 1990·Plant Physiology·S R SchnappP M Hasegawa
Nov 1, 1990·Plant Physiology·J P Spychalla, S L Desborough
Sep 1, 1991·Plant Physiology·N R Madamanchi, R G Alscher
Jun 1, 1987·Plant Cell Reports·N L Trolinder, J R Goodin

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Citations

Aug 14, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Iram SharifShahid Munir Chohan
Oct 1, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ramalingam Radhakrishnan
Aug 6, 2002·Free Radical Biology & Medicine·Lee C GarrattMichael R Davey

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