The influence of anti-endothelial/antiphospholipid antibodies on fibrin formation and lysis on endothelial cells

British Journal of Haematology
Angela M PattersonMike Greaves

Abstract

The prothrombotic mechanisms associated with antiphospholipid antibodies remain incompletely defined. Antibody binding to endothelial cells in vitro is a feature of antiphospholipid antibody-positive sera. We hypothesised that impairment of endothelium-dependent fibrinolysis by antiphospholipid/anti-endothelial antibodies is a contributory factor in the pathogenesis of thrombosis. We also aimed to confirm the displacement of annexin-V from endothelial cells and enhanced fibrin formation. Binding of immunoglobulin (Ig) from antiphospholipid antibody-positive sera to endothelial cells was examined using a cell-based enzyme-linked immunosorbent assay. Effects on fibrin formation and lysis were examined on cultured endothelial cell monolayers. Plasminogen activator inhibitor-1 (PAI-1) was assayed in supernatants. We confirmed antibody binding to endothelial cells. With four of 14 antiphospholipid antibody-positive sera there was some prolongation of fibrin clot lysis time, consistent with impairment of endothelial fibrinolytic activity. Secretion of PAI-1 was significantly correlated with clot lysis time on endothelial cell monolayers incubated with antiphospholipid/anti-endothelial antibody-positive sera, but not with control sera...Continue Reading

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Citations

Mar 7, 2008·Journal of Clinical Immunology·V SalleJ P Ducroix
Jul 10, 2009·Blood·Bill Giannakopoulos, Steven A Krilis
May 7, 2008·Current Rheumatology Reports·Richard A Marlar, Sanam Husain
Jun 19, 2007·Trends in Molecular Medicine·Kwang-Hyun BaekYong-Soo Kim
Apr 1, 2010·Lupus·R T Urbanus, B de Laat
Jan 21, 2017·F1000Research·Maria Laura Bertolaccini, Giovanni Sanna
Sep 21, 2006·Blood·Bill GiannakopoulosSteven A Krilis
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Audrey GrahamLars-Peter Erwig

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