PMID: 11316568Apr 24, 2001Paper

The influence of autologous tumor fibroblasts on the radiosensitivity of squamous cell carcinoma megacolonies

International Journal of Radiation Oncology, Biology, Physics
J KummermehrK R Trott

Abstract

To study the influence of tumor fibroblasts on radiosensitivity and stem cell fraction of tumor cells in squamous cell carcinoma megacolonies by determining colony cure and clonogen survival. Murine squamous cell carcinoma cells (AT478c) grown as flat but multilayered megacolonies were co-cultured with pre-irradiated tumor fibroblasts derived from the same carcinoma, and irradiated with 1, 2, 4, or 8 fractions. Recurrent clones and their growth pattern in situ were recorded. From megacolony cure data and clonogen survival data, the clonogen number and the parameters of cellular radiosensitivity were calculated. The curability of the co-cultured megacolonies, as determined by TCD50 values, was significantly increased compared to the megacolonies without fibroblasts (p < 0.01). Both the megacolony cure and clonogen survival data suggested a decrease of the clonogen fraction in the co-cultured megacolonies. The presence of tumor fibroblasts increases megacolony radiosensitivity. This is due to a decrease in the fraction of clonogens in the tumor megacolony, apparently caused by a downregulation of the stem cell fraction of the tumor cells.

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Citations

Feb 28, 2003·International Journal of Radiation Oncology, Biology, Physics·Rafal TarnawskiKrzysztof Skladowski
Jun 28, 2003·International Journal of Radiation Oncology, Biology, Physics·Jack F FowlerDavid J Brenner
Mar 14, 2012·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Stacy R SmithJeanne M Novak
May 14, 2008·International Journal of Radiation Oncology, Biology, Physics·Yuka NakazawaShunichi Yamashita

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