The influence of carbon sources and morphology on nystatin production by Streptomyces noursei

Journal of Biotechnology
Einar JonsbuJ Nielsen

Abstract

Carbon source nutrition and morphology were examined during cell growth and production of nystatin by Streptomyces noursei ATCC 11455. This strain was able to utilise glucose, fructose, glycerol and soluble starch for cell growth, but failed to grow on media supplemented with galactose, xylose, maltose, sucrose, lactose and raffinose. Utilisation of glucose had a negative influence on production of nystatin independent of the specific growth rate when phosphate and ammonium was in excess. Consumption of carbon sources was related to the specific growth rate. S. noursei ATCC 11455 formed mainly mycelial clumps during cultivation, while pellet growth dominated the culture of the morphologically altered high producing mutant S. noursei NG7.19. When the pellet size increased above a critical size, cell growth and nystatin production terminated. Fluorescent staining of hyphae revealed that this coincided with loss of activity inside the core of the pellets, probably due to diffusion limitation of oxygen or other nutrients.

References

Mar 1, 1985·Canadian Journal of Microbiology·M L DeklevaW R Strohl
Jan 1, 1971·Zeitschrift für allgemeine Mikrobiologie·E T Nikitina, L V Kalakoutskii
Jun 1, 1995·Journal of Industrial Microbiology·I KojimaA L Demain
Jan 1, 1951·Proceedings of the Society for Experimental Biology and Medicine·E L HAZEN, R BROWN
Apr 15, 1990·Biotechnology and Bioengineering·S E Vecht-LifshitzS Braun

❮ Previous
Next ❯

Citations

Feb 9, 2005·Applied Microbiology and Biotechnology·Espen Fjaervik, Sergey B Zotchev
Aug 22, 2009·Journal of Industrial Microbiology & Biotechnology·Byung-Gyun KimEung-Soo Kim
Nov 28, 2012·Journal of Industrial Microbiology & Biotechnology·Kamaleshwar P SinghSameer Jadhav
Dec 10, 2003·Current Opinion in Microbiology·Klas Flärdh
Jul 29, 2010·The Journal of Antibiotics·Sergio SánchezBeatriz Ruiz
Jun 20, 2012·Preparative Biochemistry & Biotechnology·Hulya Ayar KayalıLeman Tarhan
Apr 29, 2008·Applied and Environmental Microbiology·Angel MantecaJesus Sanchez
Jan 13, 2010·European Journal of Mass Spectrometry·Ales UlrychVladimir Havlicek
Jun 7, 2011·Journal of Industrial Microbiology & Biotechnology·Kamaleshwar P SinghSameer Jadhav
Nov 3, 2017·Antonie van Leeuwenhoek·Dino van Dissel, Gilles P van Wezel
Jan 15, 2017·Bioresource Technology·Punit Kumar, Kashyap Kumar Dubey
Apr 21, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Buse CinarSedef Tunca
Jun 16, 2010·Journal of Bioscience and Bioengineering·Chun-Chong FuWen-Teng Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.