PMID: 36911Jun 2, 1979

The influence of charge on phosphatidic acid bilayer membranes

Biochimica Et Biophysica Acta
H Eibl, Alfred Blume

Abstract

A complete titration of phosphatidic acid bilayer membranes was possible for the first time by the introduction of a new anaologue, 1,2-dihexadecyl-sn-glycerol-3-phosphoric acid, which has the advantage of a high chemical stability at extreme pH values. The synthesis of the phosphatidic acid is described and the phase transition behaviour in aqueous dispersions is compared with that of three ester phosphatidic acids; 1,2-dimyristoyl-sn-glycerol-3-phosphoric acid, 1,3-dimyristoylglycerol-2-phosphoric acid and 1,2-dipalmitoyl-sn-glycerol-3-phosphoric acid. The phase transition temperatures (Tt) of aqueous phosphatidic acid dispersions at different degrees of dissociation were measured using fluorescence spectroscopy and 90 degrees light scattering. The Tt values are comparable to the melting points of the solid phosphatidic acids in the fully protonated states, but large differences exist for the charged states. The Tt vs. pH diagrams of the four phosphatidic acids are quite similar and of a characteristic shape. Increasing ionisation results in a maximum value for the transition temperatures at pH 3.5 (pK1). The regions between the first and the second pK of the phosphatidic acids are characterised by only small variations in th...Continue Reading

References

May 21, 2011·European Biophysics Journal : EBJ·Patrick GaridelAlfred Blume
Jan 1, 1981·Progress in Biophysics and Molecular Biology·P J Quinn
Oct 8, 1990·Biochimica Et Biophysica Acta·G Cevc
Mar 29, 2001·Chemistry and Physics of Lipids·T PottE J Dufourc
Nov 12, 2002·Chemistry and Physics of Lipids·Geoffrey S Hird, Mark W Grinstaff
Sep 24, 2013·PloS One·Gerd Olofsson, Emma Sparr
Jun 6, 2002·Chemistry and Physics of Lipids·Rumiana Koynova, Martin Caffrey
Apr 7, 1982·Biochimica Et Biophysica Acta·D BachB A Sela
Oct 19, 1979·Biochimica Et Biophysica Acta·Alfred Blume
Aug 23, 1979·Biochimica Et Biophysica Acta·K HarlosH Eibl
Dec 21, 1981·Biochimica Et Biophysica Acta·R Sundler, D Papahadjopoulos
Nov 3, 2011·Chemical Communications : Chem Comm·Emily K Perttu, Francis C Szoka

Citations

Jul 9, 1975·Journal of the American Chemical Society·H J Galla, E Sackmann
May 7, 1971·Biochemical and Biophysical Research Communications·E Oldfield, D Chapman
Jun 5, 1973·Biochemistry·E J Shimshick, H M McConnell
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·P OverathW Stoffel
Dec 1, 1970·Chemistry and Physics of Lipids·A J Slotboom, P P Bonsen
Feb 1, 1969·Journal of Colloid and Interface Science·H EiblL L Van Deenen

Related Concepts

Tissue Membrane
Glycerin
Fluorescence Spectroscopy
Phosphoricum acidum, phosphoric acid, Homeopathic preparation
Light
Phosphatidic Acid
Calorimetry, Differential Scanning
PH.3
Esters
Membrane Fluidity

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