The influence of chitin on the immune response to the house dust mite allergen Blo T 12

International Archives of Allergy and Immunology
J ZakzukL Caraballo

Abstract

Information about the biological properties of Blomia tropicalis allergens is scarce. It is predicted that Blo t 12, an allergen with two described isoforms, contains a chitin-binding domain, similar to that found in peritrophins. Th2 adjuvant properties have been described for chitin. Therefore, it is feasible that binding to this carbohydrate influences its allergenicity. We aimed to evaluate the chitin-binding activity of Blo t 12 isoallergens and its effect on airway inflammation and antibody responses in a murine model of allergen sensitization. Chitin-binding assays were conducted with the recombinant isoallergens Blo t 12.0101 and Blo t 12.0102. BALB/c mice were sensitized via i.p. with any of the two isoforms (alone, with chitin or alum) and then challenged intranasally. Methacholine-induced bronchial hyperreactivity was tested by whole-body plethysmography and lung sections were stained with hematoxylin and eosin and periodic-acid Schiff. Total IgE and allergen-specific IgE, IgG1 and IgG2 levels were measured by ELISA. The two isoforms bound chitin, but Blo t 12.0101 showed a stronger binding capacity. Both isoforms induced total and allergen-specific IgE, airway hyperreactivity, bronchial inflammation and mucus secret...Continue Reading

Citations

May 1, 2017·International Archives of Allergy and Immunology·Eduardo Santos da SilvaFatima Ferreira
Sep 15, 2016·Molecular Nutrition & Food Research·Oliver SchlüterKarl-Heinz Engel
Jan 23, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T SoongrungA Jacquet
Sep 20, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Pinya PulsawatAlain Jacquet
Nov 14, 2018·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Josefina ZakzukLuis Caraballo
Mar 19, 2014·Current Allergy and Asthma Reports·Wayne R Thomas
Oct 16, 2019·Frontiers in Immunology·Sandra CoronadoLuis Caraballo
Jun 7, 2019·Allergy, Asthma & Immunology Research·Nathalie AcevedoLuis Caraballo
Nov 17, 2019·Food Research International·Dele RaheemConrado Carrascosa
Dec 30, 2020·International Journal of Molecular Sciences·Velky AhumadaLuis Caraballo
Nov 14, 2020·Carbohydrate Polymers·Mikhail V TsurkanHermann Ehrlich
May 7, 2020·The World Allergy Organization Journal·Luis CaraballoAlexander Karaulov
Jun 3, 2021·International Journal of Molecular Sciences·Jarmila ČelakovskáCtirad Andrýs
Aug 23, 2021·The Journal of Allergy and Clinical Immunology·Huey-Jy HuangSusanne Vrtala
Sep 30, 2019·International Archives of Allergy and Immunology·Eduardo Santos da SilvaNeuza Maria Alcantara-Neves

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