The influence of contact-inhibited growth and of agents which alter cell morphology on the levels of G- and F-actin in cultured cells

Experimental Cell Research
C S HeacockJ R Bamburg

Abstract

The amounts of G-actin and F-actin were measured in cultured cells grown under various conditions. The percent of total actin as F-actin in monolayer cultures of asynchronous cells was 72.4% in Chinese hamster ovary (CHO) cells, 57.7% in HeLa cells, 69.8% in V79 cells, and 79.5% in 1080 cells. Actin comprises 2.4-3.1% of the total protein in these cell lines. Treatment of cells with 20 microM cytochalasin B (CB) caused different cytological effects from treatment with 10 microM colchicine, but the effects characteristic of each drug were observed throughout the range of cell lines used. Of the five cell lines treated with CB only the V79 and CHO cells showed a decrease (5-8%) in the level of F-actin. Colchicine treatment of HeLa cells resulted in a 13% increase in the percent F-actin, but similar treatment of CHO cells caused no significant change in F-actin. Therefore, a change in the steady state level of F-actin is not necessary for the observed cell shape change. The F-actin levels in CHO cells treated with 7 mM procaine decreased from 72 to 65% over the first 15 min of exposure, a time during which the cells rounded. After continuous exposure of the cells to procaine for 1 h, the F-actin percentage returned to control leve...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L CarlssonU Lindberg
Jul 1, 1977·The Journal of Cell Biology·S E Hitchcock
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·V G RiddleA B Pardee
Jul 1, 1978·The Journal of Cell Biology·R W RubinE Clements
Nov 1, 1974·Analytical Biochemistry·T W Houk, K Ue
Aug 1, 1983·Experimental Cell Research·C S Heacock, J R Bamburg
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M VerderameR Pollack
May 15, 1981·European Journal of Biochemistry·V PribludaA Rotman
Nov 1, 1983·Analytical Biochemistry·C S Heacock, J R Bamburg
Jan 1, 1983·Molecular and Cellular Biology·J Tannenbaum, G C Godman
Apr 1, 1982·The Journal of Cell Biology·I Blikstad, L Carlsson
Feb 1, 1983·Molecular and Cellular Biology·S R FarmerS Penman
Feb 23, 1981·FEBS Letters·L Carlsson, I Blikstad

❮ Previous
Next ❯

Citations

Mar 31, 2004·Experimental Cell Research·Michel Schmitt-Ney, Joel F Habener
Dec 1, 1987·The Journal of Cell Biology·J R Bamburg, D Bray
Mar 12, 2009·PloS One·Stefan A KoestlerJ Victor Small
Apr 27, 2010·PloS One·Igor R KuznetsovMicah Dembo
Sep 8, 2011·Journal of Cellular Biochemistry·Galyna KlevetaKatarzyna Kwiatkowska
Jan 1, 1993·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D A DuBose, R Haugland
Jul 23, 2014·Current Biology : CB·Pinar S GurelHenry N Higgs
Aug 31, 1999·Biophysical Journal·V C AbrahamF Lanni
Dec 13, 1988·Biochemistry·K A GiulianoJ R Bamburg
Jun 5, 2016·Biochemistry. Biokhimii︠a︡·V MisicY Haj-Ahmad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.