PMID: 44594Nov 1, 1979

The influence of cytochalasin B, colchicine, and vinblastine on the attachment of Entamoeba histolytica to glass surfaces (author's transl)

Zeitschrift Für Parasitenkunde
R Michel, R Hohmann

Abstract

The attachment of E. histolytica trophozoites from monoxenic TPS-medium to glass surfaces was studied at different temperatures and pH values and in the presence of cytochalasin B, colchicine, and vinblastine. At pH 7.0 and at 36 degrees C optimal adhesion rates of the amebae were observed in the range from 80% to 95%. Marked inhibition of adhesion was noted at higher concentrations of cytochalasin B. The adhesion rate was reduced to 50% by 50 micrograms/ml CB. Higher dosages successively prevented nearly all adhesion. Colchicine was without any effect. Vinblastine had a certain low effect but only at very high dosage. The observations are consistent with the assumption that the adhesion process is at least partially mediated by cytochalasin-sensitive contractile microfilaments, whereas microtubuli are obviously not involved in the cell-substrate adhesion.

Citations

Jun 1, 1978·The Journal of Cell Biology·R D Berlin, J M Oliver
Feb 1, 1975·International Journal for Parasitology·P Cappuccinelli, L Varesio
Jun 21, 1974·Zeitschrift Für Parasitenkunde·R Michel, E Schupp
Feb 1, 1974·The Journal of Cell Biology·H L Malech, T L Lentz
Jun 1, 1971·The Journal of Cell Biology·B S SpoonerN K Wessells
Jan 15, 1971·Science·N K WessellsK Yamada
Oct 1, 1964·The Journal of Cell Biology·V T NACHMIAS

Related Concepts

Entamoeba histolytica
Glass (Substance)
Adhesiveness
Colchicine, (R)-Isomer
Hydrogen-Ion Concentration
Gastrozepin Brand of Vinblastine Sulfate
Metazoa
Cytochalasin B

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