The influence of DNA structure on the in vitro stimulation of murine lymphocytes by natural and synthetic polynucleotide antigens

Cellular Immunology
J P MessinaD S Pisetsky

Abstract

Although DNA is generally considered to be a poor immunogen, recent evidence suggests that DNA from various species differ in their immunological activity and that bacterial DNA can induce the in vitro proliferation of normal murine B cells. To delineate structural features of DNA associated with mitogenic activity, the response of murine lymphocytes to various natural and synthetic polynucleotides was determined. Both ss and dsDNA from two different bacterial strains were equally effective in inducing proliferation. This response was independent of adenosine methylation, since DNA from dam- Escherichia coli stimulated proliferation. Among the synthetic polymers tested, only the duplexes poly(dG).poly(dC), and poly(dG.dC) were mitogenic, while polymers containing dA, dT, or dI alone or in combination with dG and dC were inactive. The mitogenic activity of poly(dG.dC) was eliminated, however, upon substitution of rG for dG or 5medC for dC. The mitogenic activity did not require high molecular weight DNA since active polymers ranged in size from approximately 260 to 800 base pairs. In addition, E. coli DNA fragments of 50-300 and 125-600 bases were mitogenic. Together, these data suggest that the mitogenic activity of DNA is depe...Continue Reading

Citations

Apr 1, 1996·The Journal of Experimental Medicine·G S GilkesonD S Pisetsky
Apr 1, 1995·Immunological Reviews·D M Klinman, A D Steinberg
Nov 27, 1995·Annals of the New York Academy of Sciences·D S PisetskyM D Halpern
Feb 28, 2002·Annual Review of Immunology·Arthur M Krieg
Mar 1, 1995·The Journal of Clinical Investigation·G S GilkesonD S Pisetsky
Sep 1, 1996·The Journal of Clinical Investigation·H LiangP E Lipsky
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·B HanssP E Klotman
Aug 28, 2004·Cellular Immunology·Weiwen JiangDavid S Pisetsky
Sep 2, 2016·Chemical Reviews·Sachini U SiriwardenaAshok S Bhagwat
Jul 27, 2001·International Immunology·Y ChenD J Berg
Jan 11, 2005·American Journal of Respiratory and Critical Care Medicine·Kenichiro SuzukiHirotoshi Nakamura
Nov 1, 1995·Journal of Clinical Immunology·A M Krieg
Aug 17, 2000·Springer Seminars in Immunopathology·D S Pisetsky
Jan 16, 2019·Gene Therapy·Rajvinder KardaSimon N Waddington
Mar 30, 2000·The Journal of Gene Medicine·A M Krieg
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa B UccelliniGregory A Viglianti
Jan 1, 1995·Antisense Research and Development·D S Pisetsky

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