PMID: 11345437May 10, 2001Paper

The influence of downstream protein-coding sequence on internal ribosome entry on hepatitis C virus and other flavivirus RNAs

RNA
Rene RijnbrandStanley M Lemon

Abstract

Some studies suggest that the hepatitis C virus (HCV) internal ribosome entry site (IRES) requires downstream 5' viral polyprotein-coding sequence for efficient initiation of translation, but the role of this RNA sequence in internal ribosome entry remains unresolved. We confirmed that the inclusion of viral sequence downstream of the AUG initiator codon increased IRES-dependent translation of a reporter RNA encoding secretory alkaline phosphatase, but found that efficient translation of chloramphenicol acetyl transferase (CAT) required no viral sequence downstream of the initiator codon. However, deletion of an adenosine-rich domain near the 5' end of the CAT sequence, or the insertion of a small stable hairpin structure (deltaG = -18 kcal/mol) between the HCV IRES and CAT sequences (hpCAT) substantially reduced IRES-mediated translation. Although translation could be restored to both mutants by the inclusion of 14 nt of the polyprotein-coding sequence downstream of the AUG codon, a mutational analysis of the inserted protein-coding sequence demonstrated no requirement for either a specific nucleotide or amino acid-coding sequence to restore efficient IRES-mediated translation to hpCAT. Similar results were obtained with the s...Continue Reading

References

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Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J H HanM Houghton
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Citations

Jul 5, 2005·Comptes rendus biologies·Andrey V PisarevChristopher U T Hellen
Apr 16, 2004·Virus Research·Beatriz María García-MontalvoRosa M del Angel
Nov 20, 2002·Journal of Virology·Keril J BlightCharles M Rice
May 14, 2003·Journal of Virology·Lucy P BealesDavid J Rowlands
Feb 20, 2009·Journal of Negative Results in Biomedicine·Ebenezer TumbanWilliam B Lott
Feb 20, 2009·Genome Biology·Buyong Ma, Ruth Nussinov
Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Adrian M Di BisceglieCharles M Rice
Feb 1, 2002·FEBS Letters·Katerina I KalliampakouPenelope Mavromara
May 12, 2007·Analytical Biochemistry·Rémi FromentinDenis Leclerc
Mar 29, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rene RijnbrandAnnette Martin
Feb 10, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jean-Michel Pawlotsky, John G McHutchison
May 18, 2018·Journal of Virology·A L BaronC Baechlein
Sep 25, 2015·Journal of Virology·Caroline MarnataAnnette Martin
Nov 15, 2003·The Journal of Biological Chemistry·Srinivas Garlapati, Ching C Wang
Mar 11, 2006·The Journal of General Virology·Louisa S ChardGraham J Belsham

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