The influence of electrostatic interactions on the detection of heme-globin complexes in ESI-MS

Journal of the American Society for Mass Spectrometry
A Schmidt, M Karas

Abstract

The heme-globin complexes of hemoglobin and myoglobin are investigated in positive-ion mode and negative-ion mode using a nano-ESI source coupled to a quadrupole ion trap MS and an orthogonal time-of-flight MS. The extent of dissociation of these noncovalent complexes upon collisional activation and thus their gas-phase stability is strongly dependent on the polarity of the ESI-MS experiment as well as on the charge of the prosthetic group (ferri-heme [Fe3+-heme]+ vs. ferro-heme [Fe2+-heme]+/-0). The results clearly point to the important role of electrostatic interactions on the gas phase stability of noncovalent complexes and therefore the ion signals observed in ESI-MS experiments.

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Citations

Oct 13, 2011·Journal of the American Society for Mass Spectrometry·Lee Chuin ChenKenzo Hiraoka
May 15, 2013·Journal of the American Society for Mass Spectrometry·Modupeola A Sowole, Lars Konermann
Jun 28, 2005·Journal of the American Society for Mass Spectrometry·Guillaume ChevreuxH Lee Sweeney
Nov 4, 2008·Journal of the American Society for Mass Spectrometry·Noemi CzuczyZoltan Takats
May 15, 2003·Journal of the American Society for Mass Spectrometry·Sonya M Clark, Lars Konermann
Dec 14, 2011·Journal of Medicinal Chemistry·Hannah J MapleJohn Crosby
Feb 16, 2005·Journal of Mass Spectrometry : JMS·Daisuke NoharaKoji Takeda
May 28, 2014·Rapid Communications in Mass Spectrometry : RCM·Hannah J MapleRebecca J Burnley
Dec 7, 2010·Biochemical and Biophysical Research Communications·Young Hye KimYoung Mok Park
Feb 9, 2017·Biochemistry·Madeline E KavanaghChris Abell
Dec 18, 2001·Rapid Communications in Mass Spectrometry : RCM·P A ChrismanS A McLuckey
Feb 22, 2002·Journal of Mass Spectrometry : JMS
Sep 25, 2003·Journal of Mass Spectrometry : JMS·Maria Samalikova, Rita Grandori
Sep 4, 2010·European Journal of Mass Spectrometry·Magdalena Frańska
Dec 7, 2005·Rapid Communications in Mass Spectrometry : RCM·Kevin J Mark, D J Douglas
Mar 20, 2003·Journal of Mass Spectrometry : JMS·Martijn W H PinkseAlbert J R Heck
Oct 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bright D DanquahMichael O Glocker
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Atim A EnyenihiRoman A Zubarev

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