PMID: 3765370Sep 1, 1986Paper

The influence of extracellular and phagolysosomal pH changes on the bactericidal activity of bovine neutrophils against Staphylococcus aureus

Veterinary Immunology and Immunopathology
N CravenF J Bourne

Abstract

The influence of the pH of suspending medium on bovine neutrophil (PMN) function was assessed in tests of phagocytosis and killing of Staphylococcus aureus. Intracellular killing was markedly inhibited by moderate extracellular acidification whereas phagocytosis was little affected, except at the lowest pH level (pH 5.0). The killing of S. aureus by extracts of isolated PMN lysosomal granules showed a similar pH dependence and was optimal at pH levels above neutrality. Survival of S. aureus within PMN from different cows varied significantly and the relative differences in PMN bactericidal efficiency were maintained at all pH levels. The acidification of extracellular medium during incubation which resulted from metabolic activity of the PMN themselves, increased with increasing ratios of bacteria:PMN and varied significantly among cows. Addition of methylamine (10 mM) to elevate phagolysosomal pH inhibited phagocytosis and had no effect on intracellular survival of S. aureus. However, a lower concentration (1.5 mM) did not affect phagocytosis, but reduced bacterial survival without altering the relative differences in efficiency of PMN from different cows. It is suggested that the acidity of the extracellular medium may both r...Continue Reading

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Citations

Oct 16, 2001·Antimicrobial Agents and Chemotherapy·M Maurin, D Raoult
Oct 8, 2004·Critical Care : the Official Journal of the Critical Care Forum·John A KellumJinyou Li
Apr 1, 1989·Burns : Journal of the International Society for Burn Injuries·R Bjerknes, H Vindenes
Mar 13, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven L PercivalEmma J Woods
Nov 30, 2019·Parasitology Research·Ershun ZhouAnja Taubert
Mar 6, 2021·Frontiers in Public Health·Jesús Chávez-ReyesBruno A Marichal-Cancino

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