PMID: 9170163Feb 1, 1997Paper

The influence of extracellular matrix proteins on cutaneous and uveal melanocytes

Pigment Cell Research
S HedleyS MacNeil

Abstract

Cutaneous and ocular melanocytes are routinely cultured in complex mitogen-rich media. The physiological regulation of melanocyte proliferation and differentiation is not yet fully defined and this study summarises several separate lines of evidence which suggest that, in vivo, some of the signals required for melanocyte proliferation and differentiation may derive from extracellular matrix (ECM) proteins adjacent to these cells. Culture of cutaneous and uveal melanocytes on cell-derived and individual ECM proteins was found to influence cell morphology with such effects being most noticeable in mitogen-deficient media. Similarly, cell-derived and individual ECM proteins increased tyrosinase activity in normal cutaneous melanocytes and effects of these ECM proteins were seen most consistently in mitogen-deficient media. Uveal melanocytes (as has been reported for cutaneous melanocytes) showed preferential attachment to fibronectin over other ECM substrates. This attachment was particularly sensitive to drugs which affected intracellular calcium or calmodulin activity. Acute addition of fibronectin to coverslips of uveal melanocytes loaded with Fura-2 produced an acute and transient increase in intracellular calcium which was mo...Continue Reading

References

Aug 1, 1988·Journal of Cellular Physiology·M RansonR S Mason
Feb 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B A GilchrestM Yaar
Oct 1, 1993·The Journal of Investigative Dermatology·J G MorelliD A Norris

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Citations

Jun 15, 1999·Medical & Biological Engineering & Computing·K NakazawaO Damour
Jul 3, 2015·Diagnostic Pathology·Piotr DonizyRafal Matkowski
Dec 3, 1999·The British Journal of Dermatology·M NeitmannM Tronnier
Jun 26, 2018·Pigment Cell & Melanoma Research·Zeinab KasraianHamid Reza Rezvani

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