PMID: 6168612Jan 1, 1981Paper

The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue

Histochemistry
B HølundI Clemmensen

Abstract

The influence of fixation and tissue preparation on the immunohistochemical localization of human fibronectin in gastrointestinal tract tissue has been examined using indirect immunoperoxidase technique. The most optimal staining result with strong intensity and well defined localization was obtained on frozen sections of unfixed material. Nearly identical results with improved morphology were obtained when staining paraffin sections of tissue fixed in 96% ethanol, 96% + 1% acetic acid and absolute acetone. All other fixatives tested, 10% neutral buffered formalin. Lillie's AAF, Bouin's fixative, Clarke's fixative, 4% formaldehyde, 4% formaldehyde + 0.5% cetylpyridiniumchloride (F-CPC), 4% formaldehyde +0.1% glutaraldehyde gave unsatisfactory results. However, proteolytic digestion with pepsin of paraffin sections prior to staining of buffered formalin and F-CPCfixed material gave results comparable with those obtained on unfixed frozen sections are regards definition of the staining whereas staining intensity was decreased in some degree. No improvement was observed when using proteolytic digestion of tissue fixed in other fixatives.

References

Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K M YamadaI Pastan
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger
Jun 20, 1978·Annals of the New York Academy of Sciences·E LinderA Vaheri
Sep 1, 1979·Arthritis and Rheumatism·S M CooperF P Quismorio
Nov 1, 1979·Archives of Dermatological Research·J R CouchmanW E Parish
Jun 1, 1978·The Journal of Experimental Medicine·E EngvallE J Miller
Apr 1, 1978·The Journal of Experimental Medicine·S Stenman, A Vaheri
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
May 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·S NiewiarowskiJ F Mustard
Nov 1, 1980·Journal of Clinical Pathology·A J DixonJ O McGee
Jan 1, 1982·Arthritis and Rheumatism·I Clemmensen, R B Andersen

❮ Previous
Next ❯

Citations

Jan 1, 1991·Archives of Orthopaedic and Trauma Surgery·G ScheelP Grau
Jan 1, 1986·Archives of Oto-rhino-laryngology·L UhrenholtL Christensen
Jan 1, 1984·Rheumatology International·V MaystonP A Revell
Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·L ChristensenI Clemmensen
Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·L ChristensenI Clemmensen
Nov 21, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Bruce A PfefferStephen P Bartels
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·S R Gordon
May 13, 1999·Progress in Histochemistry and Cytochemistry·K Ostergaard, D M Salter
Jun 11, 1988·British Medical Journal·J JungeT Horn
May 1, 1989·Journal of Clinical Pathology·A J d'Ardenne
Jan 1, 1988·Acta histochemica·C PfisterD Stiller
Nov 1, 1986·Scandinavian Journal of Immunology·G Baatrup, S E Svehag
Jan 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J JungeP Christoffersen
Jan 1, 1984·Differentiation; Research in Biological Diversity·J ParankoJ M Foidart
Feb 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·J SelmerI Clemmensen
Oct 29, 2009·The American Journal of Gastroenterology·Paolo SorrentinoRaffaela Vecchione
Jul 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·B Harrach, H Robenek
Jan 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·B KaesbergH Robenek
Apr 1, 1985·The Journal of Pathology·P BirembautJ M Foidart
Apr 1, 1984·The Journal of Pathology·A J D'Ardenne, J O McGee
May 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·B HølundH Lyon
Jan 1, 1983·Acta Neuropathologica·N KochiT Itagaki
Apr 1, 1983·Arthritis and Rheumatism·I ClemmensenR B Andersen
Nov 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·M Jacobsen, G K Jacobsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.