The influence of glycol methacrylate (GMA) and paraffin embedding on freeze substituted and fixed tissues for enzyme histochemistry

Acta histochemica
I Höpfel-Kreiner, H von Mayersbach

Abstract

The influences of paraffin and GMA-embedding on acid phosphatase, esterase and beta-glucuronidase activity of differently fixed or freeze substituted rat livers were studied. 1. Embedding generally causes a reduction of the enzyme activities but improves considerably the quality of the microscopical pictures when compared with appropriate cryostat sections. Embedding therefore may serve as a very useful tool for detail studies on the cytological level. 2. The embedding media act differently on the reactive sites: a. Paraffin causes a heavy denaturation of the enzyme activity in lysosomes but preserves the activities of the ergastoplasmic (= "microsomal") enzymes. The degree of denaturation increases with increasing embedding temperature. b. GMA-embedding delivered opposite effects by preserving lysosomal activities and quenching endoplasmic enzymes. UV-polymerization of GMA causes a general inactivation of enzymes. 3. The histochemical reactivity of substrates such as glycogen was not influenced by the embedding. However, its most natural localization is achieved by freeze drying or isopropanol freeze substitution followed by GMA-embedding.

References

Jan 1, 1978·Acta histochemica·H von Mayersbach, I Höpfel-Kreiner
Apr 17, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Buc, J C Kaplan
Nov 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L Heywood, A Blackshaw
Dec 17, 1976·Archives of Toxicology·H Mayersbach
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E AshfordM E McCully
Jan 1, 1956·Acta Anatomica·B FREDRICSSON
Apr 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M HAYASHIW H FISHMAN

Related Concepts

Acid Phosphatase
Acrylic Resins
Esterases
Exo-beta-D-Glucuronidase
Cytochemistry
Histological Techniques
Liver
Liver Glycogen
Parafilm
Polyhydroxyethyl Methacrylate

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