The influence of homogenisation conditions on biomass-adsorbent interactions during ion-exchange expanded bed adsorption

Biotechnology and Bioengineering
Jürgen J HubbuchMaria-Regina Kula

Abstract

Expanded bed adsorption (EBA) is an integrative step in downstream processing allowing the direct capture of target proteins from cell-containing feedstocks. Extensive co-adsorption of biomass, however, may hamper the application of this technique. The latter is especially observed at anion exchange processes as cells or cell debris are negatively charged under common anion exchange conditions. The restrictions observed under these conditions are, however, directly related to processing steps prior to fluidised bed application. In this study, it could be shown that the effective surface charge of cell debris obtained during homogenisation is closely related to the debris size and thus to the homogenisation method and conditions. The amount and thus effect of cells binding to the adsorbent could be significantly decreased when optimising the homogenisation step not only towards optimal product release but towards a reduction of debris size and charge. The lower size and charge of the debris results not only in a reduced retention probability but also, in a lower collision probability between debris and adsorbent. The applicability was shown in an example where the homogenisation conditions of E. coli were optimised towards EBA a...Continue Reading

References

Apr 24, 1992·Journal of Chromatography·H A Chase, N M Draeger
Dec 1, 1989·Experientia·C KrekelerJ Klein
Aug 1, 1987·Applied and Environmental Microbiology·M C van LoosdrechtA J Zehnder
Aug 1, 1994·Trends in Biotechnology·H A Chase
Jul 9, 1999·Biotechnology and Bioengineering·H M Fernandez-LahoreJ Thommes
Jan 14, 2000·International Journal of Food Microbiology·J Li, L A McLandsborough
Apr 11, 2000·Journal of Chromatography. a·H M Fernández-LahoreJ Thömmes
Jun 1, 2000·Applied and Environmental Microbiology·C J Boonaert, P G Rouxhet
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·S C Burton, D R Harding
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·U ReichertJ Thömmes
Feb 2, 2002·Journal of Chromatography. a·Maria B DainiakBo Mattiasson
Nov 15, 2002·Biotechnology and Bioengineering·Dong-Qiang LinJörg Thömmes
May 28, 2003·Biotechnology and Bioengineering·Dong-Qiang LinMaria-Regina Kula
Jul 29, 2004·Biotechnology and Bioengineering·Dong-Qiang LinJürgen J Hubbuch
Aug 28, 2004·Journal of Chromatography. a·Maria E Viloria-ColsBo Mattiasson

❮ Previous
Next ❯

Citations

Jun 9, 2005·Current Opinion in Microbiology·Karl Schügerl, Jürgen Hubbuch
Jul 4, 2009·Trends in Biotechnology·Bangaru BalasundaramDaniel G Bracewell
Oct 16, 2007·Journal of Biotechnology·B Balasundaram, S T L Harrison
Sep 8, 2011·Biotechnology and Bioengineering·Darren N NesbethEli Keshavarz-Moore
Apr 30, 2015·Biotechnology Advances·J F BuyelR Fischer
Jun 21, 2017·Biotechnology Journal·Cornelia Walther, Astrid Dürauer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.