PMID: 2508783Oct 1, 1989Paper

The influence of interferon alpha and gamma, singly or in combination on human natural cell mediated cytotoxicity

Bioscience Reports
L RawlinsonR C Rees

Abstract

The influence of interferon alpha and gamma alone or in combination on the augmentation of human natural cytotoxicity was studied. Treatment of peripheral blood lymphocytes with IFN- alpha led to a rapid augmentation of NK activity, in contrast to IFN-gamma where target cell killing was observed only following 18 hrs exposure of lymphocytes to IFN-gamma. The results of the single cell assay paralleled those obtained using the Chromium release test, but neither interferon type caused an increase in the number of target binding lymphocytes. The combined effect of IFN-alpha and IFN-gamma in stimulating human natural cytotoxicity demonstrated individual lymphocyte responses to be variable. Exposure of lymphocytes to IFN-alpha and IFN-gamma for 18 hrs prior to assay for cytotoxicity usually decreased the level of cytotoxicity compared with control values, whereas other treatment regimes gave an additive and sometimes synergistic effect. Only treatment with IFN-alpha for 18 hrs and IFN-gamma for one hr produced a synergistic response in the majority of individuals tested. We conclude from this study that individual responses to IFN-alpha and IFN-gamma alone or in combination are variable and dependent upon timing of exposure of lymph...Continue Reading

References

May 15, 1975·International Journal of Cancer. Journal International Du Cancer·R C ReesR W Baldwin
Jan 1, 1980·Annals of the New York Academy of Sciences·B PerussiaG Trinchieri
Aug 15, 1982·International Journal of Cancer. Journal International Du Cancer·F R BalkwillK H Fantes
Mar 16, 1983·Biochemical and Biophysical Research Communications·D A WeigentH M Johnson

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