The influence of intracellular lactate and H+ on cell volume in amphibian skeletal muscle.

The Journal of Physiology
Juliet A Usher-SmithChristopher L-H Huang

Abstract

The combined effects of intracellular lactate and proton accumulation on cell volume, Vc, were investigated in resting Rana temporaria striated muscle fibres. Intracellular lactate and H+ concentrations were simultaneously increased by exposing resting muscle fibres to extracellular solutions that contained 20-80 mm sodium lactate. Cellular H+ and lactate entry was confirmed using pH-sensitive electrodes and 1H-NMR, respectively, and effects on Vc were measured using confocal microscope xz-scanning. Exposure to extracellular lactate up to 80 mm produced significant changes in pH and intracellular lactate (from a pH of 7.24 +/- 0.03, n = 8, and 4.65 +/- 1.07 mm, n = 6, respectively, in control fibres, to 6.59 +/- 0.03, n = 4, and 26.41 +/- 0.92 mm, n = 3, respectively) that were comparable to those observed following fatiguing stimulation (6.30-6.70 and 18.04 +/- 1.78 mm, n = 6, respectively). Yet, the increase in intracellular osmolarity expected from such an increase in intracellular lactate did not significantly alter Vc. Simulation of these experimental results, modified from the charge difference model of Fraser & Huang, demonstrated that such experimental manoeuvres produced changes in intracellular [H+] and [lactate] comp...Continue Reading

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Citations

Sep 7, 2006·Pflügers Archiv : European journal of physiology·Juliet A Usher-SmithChristopher L-H Huang
Jan 13, 2009·Biological Reviews of the Cambridge Philosophical Society·Juliet A Usher-SmithJames A Fraser
Apr 21, 2007·The Journal of Physiology·Graham Kemp
Jan 27, 2018·Nature Communications·Chen KangRajan Sah
Jun 23, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Jeffrey M Duerr, Kristina Tucker
Jan 16, 2008·Physiological Reviews·D G AllenH Westerblad
Nov 24, 2019·Cancers·Joanna BogusławskaAgnieszka Piekiełko-Witkowska
Jul 5, 2012·Chemical Society Reviews·Stephen J Butler, David Parker

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