PMID: 8583151Nov 1, 1995Paper

The influence of medium composition on the maintenance of cytochrome P-450, glutathione content and urea synthesis: a comparison of rat and sheep primary hepatocyte cultures

Journal of Hepatology
P WattsH Grant

Abstract

Rat and sheep primary hepatocytes have been cultured in four different medium formulations: Williams' E, Chee's, Medium 199 and Modified Earle's. The total cytochrome P450 content, intracellular concentration of reduced glutathione, rate of urea synthesis and total protein content of cultures of cells from both species in each medium have been determined. Modified Earle's and Chee's medium proved to be the most favourable formulations for the culture of rat hepatocytes. After 48 h, cells cultured in Modified Earle's had significantly more cytochrome P450 and a significantly greater rate of urea synthesis than cells in any other medium. After 6 days in culture the difference in cytochrome P450 levels between rat hepatocytes in Chee's medium and those in Modified Earle's medium was abrogated. The difference in the rate of urea synthesis between rat hepatocytes cultured in each of these two media was shown to be more dependent on the medium in which the cells were maintained during the period of urea synthesis measurement than on the medium in which the cells had been previously cultured. Sheep hepatocytes cultured in Chee's medium ruptured and died within 24 h. Apart from this, sheep cells were less sensitive to changes in medium...Continue Reading

References

Jul 1, 1979·Acta Pharmacologica Et Toxicologica·I AnundiA H Stead
Jan 1, 1978·Methods in Enzymology·P MoldéusS Orrenius
Oct 1, 1990·Artificial Organs·J GerlachC Müller
Sep 1, 1988·Biochemical Pharmacology·S J DuthieM H Grant
Jan 1, 1988·Antonie van Leeuwenhoek·E CasaloneM Polsinelli
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Jun 1, 1994·Human & Experimental Toxicology·C MacDonaldH Grant

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Citations

Jan 29, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G C McKayM H Grant
Sep 11, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M GunaratnamM H Grant
Sep 28, 1999·Journal of Veterinary Pharmacology and Therapeutics·J N TetteyV Zammit
Nov 14, 2000·Tissue Engineering·K BehniaM Toner
Dec 17, 2008·Tissue Engineering. Part C, Methods·Joana P MirandaPaula M Alves
Jun 11, 2005·Journal of Gastroenterology and Hepatology·Shigeki MiyamotoToshihiro Mitaka
Nov 7, 2006·Journal of Veterinary Pharmacology and Therapeutics·I BoibessotM H Grant
Oct 13, 2006·The Journal of Pharmacy and Pharmacology·A J MeechanJ N A Tettey
Jan 22, 2014·Cytotechnology·Xiaobing GongPeter Neuhaus
Jun 12, 2004·Cell Transplantation·Konstantinos J DabosJohn N Plevris
Jan 5, 2002·In Vitro Cellular & Developmental Biology. Animal·G A HamiltonA E George
Apr 19, 2005·Journal of Biomedical Materials Research. Part a·M Helen GrantThomas Groth
Mar 1, 2003·European Journal of Gastroenterology & Hepatology·Konstantinos J DabosJohn N Plevris
May 9, 2009·Journal of Medicinal Chemistry·Geoffrey D CoxonRoger D Waigh

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