PMID: 8593283Jan 31, 1996Paper

The influence of merocyanine 540 and protoporphyrin on physicochemical properties of the erythrocyte membrane

Biochimica Et Biophysica Acta
J W LagerbergJ VanSteveninck

Abstract

The interaction of the red cell membrane with merocyanine 540 or protoporphyrin led to four phenomena, most probably interrelated. (i) The morphology changed from the normal discoid to an echinocytic form. This morphological change persisted when followed over a period of 24 h. (ii) Simultaneously, cell deformability was decreased, as revealed by viscosity measurements and a cell-filtration technique. (iii) Both drugs caused swelling of the erythrocytes in isotonic medium, due to a very-short-term increased permeability of the membrane, also for larger molecules such as lactose. The pathway of this temporary leak seems to be unrelated to the Na+/K+ -ATPase, the K+/Cl- and the Na+/K+/Cl- cotransport systems, the Ca2+-activated Gardos pathway, the oxidation/deformation-activated leak pathway and the so-called residual transport route. Despite the morphological changes, K+-leakage induced by mechanical stress was not increased. (iv) During osmotic swelling, the critical hemolytic volume was found to be increased in the presence of either merocyanine 540 or protoporphyrin. The increase critical volume protected erythrocytes against osmotic hemolysis.

References

Apr 1, 1989·The Journal of Cell Biology·D L Daleke, W H Huestis
Sep 11, 1986·Biochimica Et Biophysica Acta·B IsomaaA C Engblom
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M P Sheetz, S J Singer
May 1, 1967·Biochemical Pharmacology·J van SteveninckH L Booij
Jun 1, 1993·Photochemistry and Photobiology·E Ben-HurY Granot

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Citations

Dec 6, 2014·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Satoshi KawamuraAlan G Fantel

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