PMID: 20650148Oct 1, 1995Paper

The influence of nitrite, nitrate and nitric oxide on ammonia use and urea production in primary cultures of sheep hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
W KarlikM Wiechetek

Abstract

Inorganic nitrite introduced into the living organism is rapidly converted into nitrate and nitric oxide (NO). It is known that nitrite decreases ammonia use and urea formation in vitro and it seems that nitrite also influences these processes in vivo. However, the mechanism underlying this effect is not known. Therefore, we decided to compare the influence of sodium nitrite (NaNO(2)), sodium nitrate (NaNO(3)) and NO on ammonia use and ureagenesis in monolayer cultures of sheep hepatocytes during 18 hr of cultivation. It was found that 0.5 and 2.5 mmNaNO(2) significantly reduced ammonia use in a dose-dependent manner for the first 6 hr of incubation; at higher concentrations (2.5 mm), it also decreased urea formation. Also, the presence of nitrite did not affect the lactate dehydrogenase (LDH) activity in the medium which indicates that the nitrite effect did not result from its cytotoxic action. NaNO(3) (0.5 and 2.5 mm) did not induce any changes in ammonia use and urea synthesis in hepatocytes. With sodium nitroprusside (SNP) (0.001, 0.01, 0.1, 0.5 and 1.0 mm) a decrease of ammonia use and urea production was observed corresponding to the nitrite effect, but contrary to nitrite exposure these changes in metabolism were persis...Continue Reading

References

Sep 1, 1976·Anesthesiology·J H Tinker, J D Michenfelder
May 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G A van 't KloosterA S van Miert
Jul 1, 1990·Physiological Reviews·A J MeijerR A Chamuleau
Oct 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T TakanoK Nakata
Feb 28, 1986·Biochimica Et Biophysica Acta·C DemignéP Fafournoux
Apr 1, 1993·Archives of Environmental Contamination and Toxicology·M WiechetekW Souffrant
Nov 1, 1961·The Biochemical Journal·L C WANGR H BURRIS

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Citations

Apr 12, 2002·Veterinary Immunology and Immunopathology·Wim Van den BroeckBruno M Goddeeris
Jul 6, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tomoyasu ToyoizumiNaohide Kinae
Apr 4, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Juan SunJunichi Misumi

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