The influence of nonconjugative Escherichia coli plasmids on biofilm formation and resistance

Journal of Applied Microbiology
J S TeodósioF J Mergulhão

Abstract

This work describes the effects of the presence of nonconjugative plasmids in Escherichia coli cells forming biofilms on a flow cell system under turbulent conditions. The pET28 and pUC8 plasmids were separately used to transform E. coli JM109(DE3). Biofilm formation, removal and antimicrobial susceptibility to the cationic biocide benzyldimethyldodecylammonium chloride (BDMDAC) were assessed. Transformed cells formed thicker biofilms with higher cell densities, and the metabolic activity was higher whereas nontransformed cells had higher viabilities. Biocide treatment was not efficient for biofilm removal but was effective for cell killing. Biofilms formed by nontransformed cells were less affected by the treatment. Cell transformation with the tested plasmids has significant impacts on biofilm formation, cell viability, metabolic activity and resistance to biocide treatment. Our results show that in biofilm studies involving deletion/complementation experiments, a control with the strain carrying a plasmid devoid of the gene under investigation must be included so that the real effects of the genetic manipulation are not biased by the presence of the plasmid backbone. This is the first report where the presence of nonconjugat...Continue Reading

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Citations

May 3, 2013·Bioprocess and Biosystems Engineering·J M R MoreiraF J Mergulhão
Aug 3, 2013·Biofouling·Joana S TeodósioFilipe J Mergulhão
May 17, 2014·Scientifica·Luciana C GomesFilipe J Mergulhão
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Jan 23, 2020·International Journal of Molecular Sciences·Luciana GomesFilipe Mergulhão
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Nov 8, 2017·Scanning·Luciana Calheiros Gomes, Filipe José Mergulhão
Oct 31, 2019·AIMS Microbiology·Alexandra SoaresFilipe J Mergulhão
May 26, 2018·Microorganisms·Alexandra SoaresFilipe José Mergulhão
Mar 26, 2021·Microbial Biotechnology·Alberto J Martín-RodríguezUte Römling
Jun 25, 2015·Microbiology Spectrum·Laura C C Cook, Gary M Dunny
Jun 17, 2021·Biotechnology and Bioengineering·Phillip LemkeChristof M Niemeyer

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