PMID: 9172020Dec 1, 1996Paper

The influence of organic acids on the proliferation of human peripheral lymphocytes activated by concanavalin A and pokeweed mitogen

International Journal of Immunopharmacology
K D SantosM Wajner

Abstract

The present study was undertaken to assess the influence of 25 organic acids, which appear in high concentrations in tissues of patients with various organic acidaemias, on the proliferation of human peripheral lymphocytes stimulated with concanavalin A (Con A) (a T-cell activator) and pokeweed mitogen (PWM) (predominantly a B-cell activator). Mononuclear cells were cultivated in flat-bottomed 96-well microplates at 37 degrees C for 96 (Con A) or 144 h (PWM) in the presence of one mitogen at different concentrations and of one acid at doses ranging from 1 to 5 mM. Control cultures did not contain any acid. Cell reactivity was measured by the incorporation of tritiated thymidine into cellular DNA. We observed that, among the 25 acids tested, aminoadipic (AAD), 2-hydroxy-3-methylvaleric (HMV), 2-ketoisocaproic (KIC), 2-methylbutyric (MBA), propionic (PPA) and tiglic (TIG) acids strongly suppressed lymphocyte DNA synthesis in Con A-supplemented cultures, whereas in cultures stimulated with PWM, 2-ketoisovaleric (KIV) and PPA acids presented the same effect. In contrast, lactic (LAC) and pyruvic (PYR) acids activated lymphocyte DNA synthesis in cultures treated with Con A, the same effect occurring with LAC acid for PWM-stimulated ...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S E PikeG Tosato
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Citations

Mar 28, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R H LindecronaI Thorup
Dec 29, 2004·Veterinary Immunology and Immunopathology·Cathy J WalshDavid R Noyes

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