PMID: 6170337Oct 13, 1981Paper

The influence of pH on the degradation of bovine myelin basic protein by bovine brain cathepsin

Biochimica Et Biophysica Acta
J N Whitaker, J M Seyer

Abstract

The degradation of bovine myelin basic protein by bovine brain cathepsin D (ED 3.4.23.5) was studied over a pH range of 2.75 - 6.0. Throughout this pH range pepstatin, an inhibitor of cathepsin D, prevented the degradation. The degradation at a pH away from the optimum of pH 3.5 was predictably slower, but also resulted in more restricted cleavage. Above pH 4.5 bovine basic protein peptide 1 - 42 was not degraded further to peptide 1 - 36 as occurs at pH 3.5. Additionally, at pH 5.5 another fragment of basic protein, peptide 1 - 91, persisted indicating that under certain basic protein as well as basic protein peptide 43 - 169 may be cleaved in the molecular region of basic protein around the phenylalanyl-phenylalanine residues at position 88 - 89. The small amount of peptides 1 - 91 and 92 - 169 detected at pH 5.5 suggests that the bond between residues 91 and 92 in intact basic protein is a minor cleavage site. The options and variation in cleavage around residues 88 - 92 of basic protein presumably result from pH-dependent changes in conformation in the is region but could also be due to changes in conformation of cathepsin D. These results indicate that local tissue changes such a pH amy affect not only the velocity of the ...Continue Reading

References

Aug 28, 1979·Biochemical and Biophysical Research Communications·M Artman, R J Seeley
Aug 1, 1977·The Journal of Experimental Medicine·J N WhitakerR F Kibler
Apr 1, 1975·European Journal of Biochemistry·M BenuckG A Hashim
Jan 1, 1979·The Biochemical Journal·A Dionyssiou-Asteriou, E T Rakitzis
Aug 28, 1979·Biochemical and Biophysical Research Communications·S WatabeN Yago
Feb 1, 1979·Journal of Neurochemistry·J N Whitaker, J M Seyer
Dec 6, 1976·Biochemical and Biophysical Research Communications·B E Chapman, W J Moore
Jul 1, 1974·The Biochemical Journal·P R Dunkley, P R Carnegie
Jul 6, 1981·Brain Research·J N WhitakerW O Whetsell

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Citations

Nov 1, 1991·Journal of Neurochemistry·G V JohnsonJ N Whitaker
Jun 1, 1992·Journal of Neurochemistry·M Banay-SchwartzA Lajtha
Apr 5, 2011·Bioorganicheskaia khimiia·A V BachevaA G Gabibov
Sep 18, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David S Libich, George Harauz

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