PMID: 9444625Jan 28, 1998Paper

The influence of polyamines synthesis inhibition on pancreas regeneration and phospholipase D activity after acute caerulein induced pancreatitis in rats. Biochemical and ultrastructural study

Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society
G JurkowskaA Andrzejewska

Abstract

The importance of polyamines in tissue growth and regeneration was shown. The involvement of phospholipase D (PLD) activity in pancreas recovery after acute pancreatitis (AP) was postulated. Thus, the aim of present study was to evaluate: the effect of polyamines synthesis inhibition on pancreas regeneration after AP and possible relationship between polyamines metabolism and PLD activity during recovery after AP. AP was induced by s.c. injection of caerulein (Cae.) in gelatin (12 micrograms/kg; t.i.d.) during 2 days. After AP inducion rats were treated with the irreversible inhibitor of polyamines synthesis alpha-difluoromethylornithine (DFMO) and/or putrescine or saline for 2, 7 and 14 days. Pancreatic weight, total protein, enzymes, nucleic acids contents were evaluated and ultrastructural examination was performed. Also pancreatic acini were prepared and loaded with [3H] myristic acid to measure 3H phosphatidic acid (PA), a marker of PLD activity. For in vitro study the pancreatic acini from healthy rats were preincubated with 1 mM DFMO and stimulated with Cae. AP results in pancreas destruction, followed by spontaneous recovery within 14 days. We found that treatment with DFMO during AP induction did not produce more sever...Continue Reading

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