PMID: 6412688Sep 1, 1983Paper

The influence of poly(ethylene glycol) 6000 on the properties of skeletal-muscle actin

The Biochemical Journal
R L TellamP R Wills

Abstract

Poly(ethylene glycol) 6000 affected many of the properties of skeletal-muscle actin. It accelerated the rate and increased the extent of actin polymerization as measured by light-scattering and sedimentation studies respectively. Moreover, intrinsic-fluorescence measurements showed that addition of poly(ethylene glycol) 6000 decreased the rate of EDTA-induced denaturation of actin monomer and increased the temperature at which irreversible conformational changes occur in actin monomer. These effects occurred without any apparent direct binding interaction and are postulated to be a consequence of the effect of excluded volume on the thermodynamic activity of actin. A relationship based on spherical geometry was formulated which described the co-volume increment that occurs upon addition of a monomer to a long linear polymer in the presence of a space-filling macromolecule. The application of this relationship to the poly(ethylene glycol) 6000-actin system was not without assumption, but it permitted quantitative estimation of the co-volume increment which proved to be of the sign and magnitude required to explain the increased extent of actin polymerization found experimentally in the presence of various concentrations of poly(...Continue Reading

Citations

Sep 1, 2005·Annals of Biomedical Engineering·Florin DespaRaphael C Lee
Dec 22, 2007·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Chunyang ZhengZhiguo Su
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Loren StaggPernilla Wittung-Stafshede
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Nov 8, 2014·Physical Chemistry Chemical Physics : PCCP·Christopher RosinRoland Winter
Jun 1, 2013·Biophysics Reviews·Alexander ChristiansenPernilla Wittung-Stafshede
Dec 16, 1991·FEBS Letters·E GraziA Cataldi
Oct 2, 2001·The Journal of Biological Chemistry·E G YarmolaM R Bubb
Mar 15, 2006·Annals of the New York Academy of Sciences·Florin DespaRaphael C Lee
Aug 1, 1984·Journal of Muscle Research and Cell Motility·M StrömqvistV P Shanbhag
Aug 3, 1999·European Journal of Biochemistry·R AdamiE Grazi
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·A Oplatka
Jan 4, 2005·Biochemistry. Biokhimii︠a︡·N A ChebotarevaN B Livanova
Jul 17, 2010·Biochemistry. Biokhimii︠a︡·Yejing WangSen Li
Dec 17, 2014·International Journal of Molecular Sciences·Irina M KuznetsovaVladimir N Uversky
Jul 16, 2008·Progress in Biophysics and Molecular Biology·Marc S CorteseA Keith Dunker
Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Nov 15, 2020·Nature Communications·Birgit Köhn, Michael Kovermann
Oct 9, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Akira R Kinjo, Shoji Takada
Jul 11, 2006·Journal of Cell Science·Allen P Minton
Jul 20, 2001·The International Journal of Biochemistry & Cell Biology·M Al-Habori
May 21, 1997·Biophysical Chemistry·R A Lindner, G B Ralston
Feb 19, 2000·Current Opinion in Structural Biology·A P Minton
Jun 1, 1994·The International Journal of Biochemistry·N Cole, G B Ralston
Jul 25, 2003·Biophysical Chemistry·Raffaella AdamiEnrico Grazi
Jan 1, 1985·Archives of Biochemistry and Biophysics·L W NicholD J Winzor
Apr 1, 1984·Archives of Biochemistry and Biophysics·L A FahienM Marshall
Jul 5, 2005·Burns : Journal of the International Society for Burn Injuries·F DespaR C Lee
Jun 22, 2011·The Journal of Physical Chemistry Letters·Edward P O'BrienD Thirumalai
Mar 3, 2015·The Journal of Physical Chemistry. B·Jean-Yves DewavrinMichael Raghunath
Mar 8, 2011·Biochemistry·Ximena AguilarPernilla Wittung-Stafshede
Jul 13, 2006·Biochemistry·Jianxin GuoC Russell Middaugh
Oct 30, 1987·Science·E D KornD Pantaloni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.