The influence of pre-operative risk on the number of circulating endothelial progenitor cells during cardiopulmonary bypass

Cytotherapy
Yeong-Hoon ChoiThorsten Wahlers

Abstract

The number of circulating endothelial progenitor cells (EPC) depends on cytokine release and is also associated with cardiovascular risk factors. During cardiopulmonary bypass (CPB) the endothelium is the first organ to be affected by mechanical and immunologic stimuli. We hypothesized that the magnitude of EPC mobilization by CPB correlates with the pre-operative cardiovascular morbidity profile. EPC were quantified in blood samples from 30 patients who underwent cardiac surgery by magnetic bead isolation and fluorescence-activated cell sorting (FACS) analysis, based on concomitant expression of CD34, CD133 and CD309. Patients were divided into two groups based on the European System for Cardiac Operative Risk Evaluation (EuroSCORE): low risk (LR) and high risk (HR). Ten healthy volunteers served as controls. Samples were obtained before the start of CPB and at 1 and 24 h post-operatively. Plasma samples were collected for determination of release levels of cytokines and growth factors. All CPB patients showed a significantly reduced basal number of EPC compared with healthy individuals (LR 5.60 +/- 0.39/mL, HR 3.89 +/- 0.34/ mL, versus control 0.807 +/- 0.82/mL, P = 0.012 versus LR, P< 0.001 versus HR). CPB induced EPC releas...Continue Reading

References

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Citations

Mar 20, 2010·Journal of Angiogenesis Research·Aurangzeb SiddiqueChetan Varma
Jul 9, 2013·The Journal of Surgical Research·Rajika RoyChristof Stamm
Nov 12, 2015·Pediatric Cardiology·Eric J KortStefan Jovinge
Feb 4, 2014·Journal of Korean Academy of Nursing·So Young Roh, Kye Ha Kim

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