The influence of pro-longevity gene Gclc overexpression on the age-dependent changes in Drosophila transcriptome and biological functions

BMC Genomics
Alexey A MoskalevAnna V Kudryavtseva

Abstract

Transcriptional changes that contribute to the organism's longevity and prevent the age-dependent decline of biological functions are not well understood. Here, we overexpressed pro-longevity gene encoding glutamate-cysteine ligase catalytic subunit (Gclc) and analyzed age-dependent changes in transcriptome that associated with the longevity, stress resistance, locomotor activity, circadian rhythmicity, and fertility. Here we reproduced the life extension effect of neuronal overexpression of the Gclc gene and investigated its influence on the age-depended dynamics of transcriptome and biological functions such as fecundity, spontaneous locomotor activity and circadian rhythmicity, as well as on the resistance to oxidative, proteotoxic and osmotic stresses. It was shown that Gclc overexpression reduces locomotor activity in the young and middle ages compared to control flies. Gclc overexpression slowed down the age-dependent decline of locomotor activity and circadian rhythmicity, and resistance to stress treatments. Gclc level demonstrated associations with the expression of genes involved in a variety of cellular processes including Jak-STAT, MAPK, FOXO, Notch, mTOR, TGF-beta signaling pathways, translation, protein processing...Continue Reading

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Citations

Jan 6, 2018·Biomedicines·Ilya O VelegzhaninovOlesya M Vakhrusheva
Dec 7, 2018·Disease Models & Mechanisms·Matthew T PereiraLaura Palanker Musselman
May 29, 2020·Mechanisms of Ageing and Development·Zulfiia GuvatovaAlexey Moskalev
Oct 24, 2020·BMC Genetics·Tatiana V TatarinovaYuriy L Orlov

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Methods Mentioned

BETA
PCR
Illumina sequencing
RNA-Seq

Software Mentioned

PPLine
R
R ( R core Team )
Illumina HiSeq Analysis
GSEA
edgeR
R core Team
clusterProfiler
pathview
STATISTICA

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