Abstract
Different industrial processes exist to purify allogenic bone, providing safe and cleaned blocks for bone allografting. However, they often make use of chemical reagents that can be aggressive for the bone matrix. Bone samples were processed with several soaking techniques used in industry: NaHCO3, H2O2, NaOH and H2O2+NaOH combined; the consequences on the bone matrix and cytocompoatibility were evaluated on femoral heads from osteoarthritic patients. Alterations of matrix were searched by histochemistry, atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cytocompatibility was evaluated by coculturing human osteoblast-like cells (SaOS-2) on bone slices. Collagen fibers were dramatically altered at the surface of bone treated with H2O2, NaOH (and their association), but not with NaHCO3. A marked reduction in the number of hydroxyapatite crystals was observed on the trabecular surfaces by TEM and morphological changes were evidenced in SEM and AFM. Argyrophilic proteins of the bone matrix were removed by H2O2 and NaOH (and their association), but not by NaHCO3. As a consequence, attachment, spreading, proliferation and alkaline phosphatase activity of SaOS-2 were reduced ...Continue Reading
References
Dec 1, 1992·Journal of Periodontology·J T MellonigM P Moyer
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F P ReinholtD Heinegård
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jan 1, 1983·Metabolic Bone Disease & Related Research·A Boyde, S J Jones
Jul 1, 1994·Biomaterials·J FagesP Frayssinet
May 1, 1993·Matrix : Collagen and Related Research·M OhshimaD Fujimoto
Jun 1, 1993·Biomaterials·D ChappardA Rebel
Feb 1, 1993·The Journal of Cell Biology·R L Juliano, S Haskill
Feb 1, 1993·Journal of Virological Methods·D R Ernst, R E Race
Aug 1, 1996·Acta Orthopaedica Scandinavica·P AspenbergS Lidin
Sep 23, 1997·Mechanisms of Ageing and Development·H SuzukiY Abiko
May 7, 1998·Journal of Biomedical Materials Research·M F BasléD Chappard
Oct 31, 1998·International Orthopaedics·M AnastasescouC Delloye
Jan 12, 1999·Biomaterials·P FrayssinetJ Fages
May 18, 1999·Journal of Biomechanics·A D SpeirsL P Nolte
Feb 3, 2000·Biomaterials·M F MoreauD Chappard
Dec 18, 2001·Journal of Biomedical Materials Research·Celaletdin ErgunRobert H Doremus
Jul 11, 2002·Oral Diseases·T Katagiri, N Takahashi
Apr 2, 2003·Bone·R KlugerO Hoffmann
Apr 25, 2003·Calcified Tissue International·M F Young
Jun 18, 2003·Biomaterials·Omana A TrentzOtmar L Trentz
Nov 15, 2003·Biomaterials·D Tadic, M Epple
Jun 23, 2004·Bone·Tue HassenkamPaul K Hansma
Dec 29, 2004·Growth Factors·Di ChenGregory R Mundy
May 10, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Pamela J VandeVordPaul H Wooley
Jul 22, 2008·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·P SeiteA M Kurt
Citations
Feb 25, 2011·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·D ChappardM Audran
Mar 28, 2013·Current Osteoporosis Reports·Peter M GoveyHenry J Donahue
May 18, 2012·The Journal of Biological Chemistry·Aleksandra MieczkowskaGuillaume Mabilleau
Mar 21, 2013·Tissue Engineering. Part a·Alayna E LoiselleHenry J Donahue
Aug 21, 2013·Cell and Tissue Banking·Naili ZhangBaoxing Li
Mar 6, 2008·Cell and Tissue Banking·Miguel Gustavo Setúbal AndradeMoysés Sadigursky
Nov 27, 2007·Morphologie : Bulletin De L'Association Des Anatomistes·F Pascaretti-GrizonD Chappard
Aug 7, 2008·Journal of Biomedical Materials Research. Part a·Aline DumasDaniel Chappard
Feb 5, 2013·Artificial Organs·Long BiZhi Yuan
Aug 26, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·R GiardinoG Giavaresi
Jul 22, 2014·BioMed Research International·Andrada SerafimDaniel Chappard
Dec 17, 2008·Biomedical Materials·Bernard GuillaumeDaniel Chappard
Aug 12, 2018·Pharmaceutics·Riccardo FerraciniGiuseppe Perale
Nov 12, 2020·Polymers·Hafiz Muhammad BasitNauman Rahim Khan
Apr 5, 2021·Morphologie : Bulletin De L'Association Des Anatomistes·L RonyD Chappard
Jun 29, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Miguel Gustavo Setúbal AndradeMoysés Sadigursky