The influence of the crystallinity of electrostatic spray deposition-derived coatings on osteoblast-like cell behavior, in vitro

Journal of Biomedical Materials Research. Part a
M C SiebersJ Jansen

Abstract

This article describes the influence of the crystallinity of carbonate apatite (CA) coatings on osteoblast-like cell behavior. Porous CA coatings were produced with electrostatic spray deposition (ESD), and subsequently, received heat treatments of 400, 500, or 700 degrees C to induce various coating crystallinities. As a result, an amorphous calcium phosphate (ACP), a crystalline CA (CCA), and a crystalline carbonated hydroxyapatite (CHA) structure were formed, respectively. Uncoated titanium substrates served as the control group. After seeding rat osteoblast-like cells, the initial cell attachment was similar between the groups, and approached 100% after 6 h. Between the various coatings, no differences were observed for proliferation, differentiation, or mineralization. However, proliferation of the osteoblast-like cells was lower on all coated substrates after longer culture periods, compared to the uncoated substrates, while at the same time differentiation was stimulated. Furthermore, after 8 and 16 days of incubation, scanning electron microscopy showed more signs of mineralization on coated substrates, compared to the uncoated substrates. In conclusion, porous ESD-derived CA coatings have a positive effect on the in vi...Continue Reading

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Citations

May 28, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Chihiro MochizukiMitsunobu Sato
Apr 20, 2012·International Journal of Oral and Maxillofacial Surgery·Makoto HirotaIwai Tohnai
Jul 22, 2008·Biomaterials·Thanaphum OsathanonCecilia M Giachelli
Aug 1, 2015·Journal of Tissue Engineering and Regenerative Medicine·Samuel HerbergJames J Cray
Dec 5, 2013·Stem Cells Translational Medicine·Jinling MaJeroen J J P van den Beucken

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