PMID: 4900610Dec 1, 1969Paper

The influence of the mode of nutrition on the digestive system of Ochromonas malhamensis

The Journal of Cell Biology
H J StoltzeO A Roels

Abstract

The intracellular distribution and level of acid hydrolases in Ochromonas malhamensis were studied in cells grown osmotrophically in a defined medium, in a carbon-free starvation medium, and during phagotrophy in each of these media. By cytochemical techniques, little enzymic reaction product was observed in the vacuoles of osmotrophic cells grown in the defined medium. Starved cells, however, contained autophagic vacuoles and cannibalized other Ochromonas cells. Dense enzymic reaction product was observed in the digestive vacuoles and in the Golgi cisternae of these starved cells. Moreover, starved cells and cells grown in a nutritionally complete medium ingested Escherichia coli which appeared in digestive vacuoles containing enzymic reaction product. Biochemical assays for lysosomal acid phosphatase (E.C. 3.1.3.2 orthophosphoric monoester phosphohydrolase) and acid ribonuclease (E.C. 2.7.7.16 ribonucleate nucleotido-2'-transferase) were done on Ochromonas cultures in the same experimental treatments and under identical assay conditions as the cytochemical study. During starvation, the acid hydrolase specific activities were consistently twice those found in cells grown in an osmotrophic complete medium. Ochromonas fed E. col...Continue Reading

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Citations

Jun 22, 1975·Archives of Microbiology·M Lasman, D Kahan
Jan 1, 1972·Archiv für Mikrobiologie·M C Cocucci, G Rossi
Nov 1, 1974·The Journal of protozoology·M A Rudzinska
Jan 1, 1971·Acta paediatrica Scandinavica·F SchettiniP Zizzadoro
Sep 1, 1969·British Journal of Haematology·F A Oski, H Bowman

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