The influence of the proliferating cell nuclear antigen-interacting domain of p21(CIP1) on DNA synthesis catalyzed by the human and Saccharomyces cerevisiae polymerase delta holoenzymes.

The Journal of Biological Chemistry
E GibbsJ Hurwitz

Abstract

In eukaryotes, processive DNA synthesis catalyzed by DNA polymerases delta and epsilon (pol delta and epsilon) requires the proliferating cell nuclear antigen (PCNA). It has recently been shown that in humans (h), the PCNA function, required for both DNA replication and nucleotide excision repair, can be inactivated by p21(CIP1) due to a specific interaction between hPCNA and the carboxyl terminus of p21(CIP1). In this report, we show that Saccharomyces cerevisiae (S. cerevisiae) PCNA-dependent pol delta-catalyzed DNA synthesis was inhibited less efficiently than the human system by the intact p21(CIP1) protein and was unaffected by the p21(CIP1) carboxyl-terminal peptide (codons 139-160). This species-specific response of PCNA to p21(CIP1)-mediated inhibition of DNA synthesis results from a marked difference in the ability of h and S. cerevisiae PCNA to interact with p21(CIP1). As shown by binding studies using the surface plasmon resonance technique, hPCNA binds both full-length p21(CIP1) and the p21(CIP1) peptide-(139-160) stoichiometrically with a similar affinity (KD approximately 2.5 nM) while S. cerevisiae PCNA binds p21(CIP1) with approximately 10-fold less affinity and does not interact with the p21(CIP1) peptide-(139-...Continue Reading

References

Jul 11, 1992·Nucleic Acids Research·A F Nichols, A Sancar
Sep 22, 1992·Biochemistry·M C YoungP H von Hippel
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T MatsumotoJ Hurwitz
Jan 1, 1989·Annual Review of Biochemistry·M D Challberg, T J Kelly
Jan 1, 1989·Annual Review of Cell Biology·B Stillman
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Aug 1, 1985·Molecular and Cellular Biology·B W Stillman, Y Gluzman
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J J Li, T J Kelly
Jan 1, 1995·Annual Review of Biochemistry·Z Kelman, M O'Donnell
Sep 15, 1995·The Journal of Biological Chemistry·Z Q PanJ Hurwitz
Dec 20, 1993·Journal of Molecular Biology·J Kuriyan, M O'Donnell
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Flores-RozasJ Hurwitz
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Dec 16, 1993·Nature·Y XiongD Beach
Jan 11, 1993·Nucleic Acids Research·M O'DonnellJ Hurwitz
Jan 12, 1996·Cell·D R Herendeen, T J Kelly

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Citations

Jan 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jane E LadnerZvi Kelman
May 1, 2002·The EMBO Journal·Salvador MacipStuart A Aaronson
Jun 15, 2004·Journal of Virology·Aitor G GranjaYolanda Revilla
Oct 5, 2001·Molecular and Cellular Biology·L HaracskaS Prakash
Jan 11, 2002·Molecular and Cellular Biology·Lajos HaracskaSatya Prakash
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·H WatanabeY Xiong
Nov 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ildiko UnkLajos Haracska
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G ZhangJ Hurwitz
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z KelmanJ Hurwitz
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·L HaracskaS Prakash
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Key-Sun Kim
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Nam Deuk KimYoung Hyun Yoo
May 5, 2016·Nucleic Acids Research·Amanda S AltieriZvi Kelman
Jul 22, 2008·Mutation Research·Jean-François MillauRégen Drouin
Jul 2, 2003·FEBS Letters·Jonathan B Vivona, Zvi Kelman
Jun 21, 2016·Nature Cell Biology·Panagiotis GalanosVassilis G Gorgoulis
Jul 25, 1998·The Journal of Biological Chemistry·K J GerikP M Burgers
May 3, 2002·The Journal of Biological Chemistry·Xiaoqing LuAntero G So
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·X LingA M Krensky
Jun 25, 1998·Molecular and Cellular Biology·S Waga, B Stillman
Nov 9, 2018·Frontiers in Molecular Biosciences·Amanda S Altieri, Zvi Kelman
Sep 12, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T OkuT Tsurimoto
Feb 9, 2006·Chemical Reviews·Marie L RossiRobert A Bambara
Feb 5, 2000·Current Biology : CB·M M Hingorani, M O'Donnell
Jun 28, 2021·The Journal of Biological Chemistry·Premlata KumariNarottam Acharya
Oct 6, 1998·Annual Review of Biochemistry·S Waga, B Stillman

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