The influence of the response of skin equivalent systems to topically applied consumer products by epithelial--mesenchymal interactions

Toxicology in Vitro : an International Journal Published in Association with BIBRA
L P BernhoferK M Martin

Abstract

A number of diverse in vitro model systems have been employed for the prediction of irritation potential of test articles. Monolayer systems have proven to be useful for preliminary screening but are not always capable of distinguishing mild effects or adaptable to fully formulated product. Three-dimensional reconstructed skin equivalents integrate cellular toxicity with the kinetics of exposure and absorption, serving as more realistic models; however, it is not obvious which of the three-dimensional models will give the most predictive response, and which biomarker should be used for an endpoint measurement for different groups of irritants. While evaluating these variables, we have shown that different irritants modulate various cytokine mRNA levels and secretion patterns in a time- and concentration-dependent manner that is unique to each product category. These profiles are also dependent on keratinocyte-fibroblast interactions. The most predictive combinations of model systems and biomarkers for each product category were identified following comparison to preclinical data and human in vivo skin responses. Using a panel of representative consumer products, we identified IL-1alpha, IL-1ra, IL-8 and GM-CSF release from skin...Continue Reading

References

Jan 1, 1990·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·D Anderson
Feb 1, 1995·International Journal of Immunopharmacology·M BaggioliniB Moser
Jan 1, 1995·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·M Ponec, J Kempenaar
Jul 1, 1993·The Journal of Cell Biology·H SmolaN E Fusenig
Jul 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A P WalkerJ Dupuis
Nov 1, 1996·Molecular Medicine Today·A HaradaK Matsushima
Jan 1, 1997·Archives of Toxicology. Supplement. = Archiv Für Toxikologie. Supplement·I KimberM Cumberbatch
Sep 21, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·M Constanza CamargoBarbara G Schneider
Oct 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F G BurlesonG F Gerberick
Apr 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L P BernhoferK M Martin
Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C L CannonM Klausner

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Citations

Mar 30, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Thomas WelssKlaus R Schröder
Sep 4, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J W CasasK P Coleman
Apr 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L P BernhoferK M Martin
Sep 19, 2012·Dermatology Research and Practice·Lorena S TelofskiGeorgios N Stamatas
Jul 20, 2004·Skin Pharmacology and Physiology·A zur MühlenM Hermann
Mar 20, 2015·BioMed Research International·Raif Eren AyataRoxane Pouliot
Aug 7, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Cathy RasmussenB Lynn Allen-Hoffmann
Sep 2, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ramya MallampatiMandip S Singh
Jun 1, 2012·Expert Review of Dermatology·James Varani
Jun 29, 2005·International Journal of Toxicology·UNKNOWN Cosmetic Ingredient Review Expert Panel
Mar 1, 2012·Cellular and Molecular Bioengineering·Nripen S SharmaMartin L Yarmush
Jun 1, 2004·Alternatives to Laboratory Animals : ATLA·Joseph KubilusMitchell Klausner
Oct 15, 2020·Journal of Cosmetic Dermatology·Xiao MaBo Wang
Feb 9, 2002·Science·Linda G Griffith, Gail Naughton

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